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- W133419669 abstract "Three species of Aeromonas; A. hydrophila (60 isolates), A. sobria (34isolates) and A. caviae(113isolates) were isolated from beef, milk,freshwater prawns, fish, and water from fishpond. A. hydrophila waspredominant in fish and freshwater prawn, whereas A. caviae waspredominant in the milk and water samples. A. sobria was only found infreshwater prawn, water and fish samples. The results of the study indicatedthat all the five different sources examined were potential vector forAeromonas in Malaysia. The percentage of antibiotic resistant amongAeromonas species was varying according to its origin. A. hydrophila wasmore resistant towards ampicillin, bacitracin, carbenicillin and erythromycin.Most of the A. sobria isolates were resistant to ampicillin, bacitracin,carbenicillin and streptomycin and A. caviae was resistant to ampicillin,bacitracin and carbenicillin. The multiple antibiotic resistance (MAR) index ofA. hydrophila, A. sobria and A. caviae ranged between 0.08 to 0.66, 0.16 to0.66, and 0 (zero) to 0.5, respectively. Hence, the MAR indexing of theAeromonas strains showed that more than 90% originated from high were detected in 50 of the 60 A. hydrophila isolates and 35 plasmid profileswere identified. The plasmid size ranged from 1.4 to 7.0 MDa. Plasmids of1.4 to 4.1 MDa were detected in 28 of the 34 A. sobria isolates tested, whilesix isolates were found to be plasmidless. Their plasmid patterns weregrouped into 18 patterns. Twenty-one plasmid patterns were found from A.caviae with plasmid sizes ranging from 1.4 to 8.0 MDa. In general,Aeromonas species under study harboured high number of plasmids. Theplasmid analysis indicated the presence of more than one clone with thesame antibiotic resistance patterns. The data obtained indicated that thesamples sources tested form a reservoir for multiple-resistant and plasmidcontaining Aeromonas species in the study area. The RAPO-PCR wasperformed to characterise the Aeromonas spp. by using three randomprimers (GEN26003, GEN26007, and GEN26010). Among the Aeromonasspp., A. hydrophila was found to be differentiated by the three primersdistinctively. Primer GEN26003 was the most suitable primer to differentiatethe isolates. The three primers generated polymorph isms in all 207 strains ofAeromonas species tested, producing bands ranging from 0.24 to 4.5 Kb.The RAPO profiles revealed a wide variability and no correlation with thesource of isolation. In addition, the RAPO data suggested that theAeromonas strains might have originated from diverse sources." @default.
- W133419669 created "2016-06-24" @default.
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- W133419669 date "1999-01-01" @default.
- W133419669 modified "2023-09-27" @default.
- W133419669 title "Molecular Characterisation of Aeromonas Species Isolated from Water and Selected Food Molecular Characterisation of Aeromonas Species Isolated from Water and Selected Food" @default.
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