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- W133482218 abstract "Siddiqui, Md Noman M.S., Purdue University, May 2014. Oxidative stress in algae: method development and effects of temperature on antioxidant nuclear signaling compounds. Major Professor: Paul Brown. Non-enzymatic antioxidants neutralize the oxidative stress through scavenging reactive oxygen species. The structural complexity of non-enzymatic nuclear signaling antioxidants poses are more challenges for traditional quantitative analysis as these compounds have multiple forms and structurally diverse than proteins and therefore, challenge traditional analytical techniques. Current analytical methods cannot distinguish the carotenoids, and vitamin A and D metabolites within a single sample. To find an appropriate method to measure all the nuclear signaling compounds in algae, Isochrysis sp., under thermal stress, HPLC/ESI-MS and HPLC /APPI-MS was used. Standards of those compounds were run by QQQ HPLC/ ESI-MS and HPLC /APPI-MS methods by multiple reaction monitoring to optimize the most sensitive transition ion for each compound. Organic solvents (cold acetone and petroleum ether) were used for extraction. Neither APPI nor ESI method showed the sensitivity to detect all targeted nonenzymatic compounds. ESI method detects lower number of compounds than APPI method, but the sensitivity of this method was higher than APPI. Isochrysis sp., was kept at 25 °C, 30 °C and 35 °C for 96 hrs to observe the changes of non-enzymatic antioxidants (carotenoids and vitamins) oxidative stress. Algae" @default.
- W133482218 created "2016-06-24" @default.
- W133482218 creator A5032675931 @default.
- W133482218 date "2014-01-01" @default.
- W133482218 modified "2023-09-26" @default.
- W133482218 title "Oxidative stress in algae: Method development and effects of temperature on antioxidant nuclear signaling compounds" @default.
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