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- W133510908 abstract "A wavelength interrogation-based surface plasmon resonance (SPR) biosensor has been investigated for a detection of avian influenza DNA (AI-DNA). The immobilization of oligonucleotides and hybridization reaction of AI-DNA was monitored in real time without labeling. The results showed that SPR biosensor can provide linear detection suitable for quantification of AI-DNA. This study demonstrates the potential for a simple AI virus diagnosis. Since these viruses can be transmitted into humans and often are fatal, improved AI detection is important. Since avain influenza viruses (AIVs) not only lead to economic losses but also cause a fatal disease in humans, a rapid screening of AIVs is required [1]. Various virological detection methods, involving virus isolation and RT-PCR, have been introduced for AIVs detection. However, they require relatively long measurement time, complex experimental sequences, and expensive huge facilities. To overcome these limitations, it is desirable to develop a rapid, labelfree, and highly specific detection method to identify an AIV infection. Surface plasmon resonance (SPR) is well-known as an effective technique without any labeling [2]. This method is also applicable in situ real time monitoring, because the plasmon waves can detect the DNA hybridization kinetics immediately. In this study, we employed a wavelength interrogation SPR method for a quantitative detection of AI-DNA hybridization. 1. SPR setup and materials We used three different oligonucleotides, as listed in Table 1. Target probe was originated from A avian influenza (NY/73-63-6/00(H7N2)) hemagglutinin. A gold film with a thickness of 45 nm was sputtered on a SF10 slide glass with a 5nm chromium adhesion layer. We used a custom-made Kretschmannconfiguration optical setup to detect SPR signals as shown in Fig. 1. An incidence angle was fixed at 60° for wavelength scanning from 550 to 650 nm. Two PDMS microfluidic (working and reference) channels were placed on the SPR sensor chip and all SPR measurements were carried with a flow rate of 2 l/min. The resonance wavelength was determined by fitting the reflectance curve to the second-order 16 T2B-3" @default.
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- W133510908 date "2009-05-13" @default.
- W133510908 modified "2023-09-27" @default.
- W133510908 title "Detection of Avian Influenza-DNA Hybridization using a Wavelength Interrogation-based Surface Plasmon Resonance Biosensor" @default.
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