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- W1336211 abstract "This chapter describes a mouse model of Mycoplasma genital infections. Prior screening to experimental infection is necessary to ensure that the mice are free of mycoplasmal organisms. Hormone treatment has a key role in predisposing female mice to colonization of the genital tract by various mycoplasmas, and so progesterone or oestrogen is used. Mice are used that, as a result of the screening process, are considered to be free of the mycoplasma to be inoculated. The stage of the reproductive cycle is checked, and the organisms are given at a time that coincides with the second injection of hormone. The aliquot of organisms, stored at – 70˚ C is thawed rapidly and kept on wet ice during the inoculation procedure. The ability of the mycoplasma to colonize the genital tract, the stage of the reproductive cycle and the effect of antimicrobial therapy are assessed at intervals, perhaps weekly, by taking swab specimens from the vagina for isolation of organisms and for vaginal cytology. The major pitfall of this model is usually ascribed to small animal models, namely the likelihood that it does not fully reflect the human situation. However, from the point of view of assessing the effect of antibiotics, the results obtained match the expectations, namely that tetracyclines are effective in eliminating mycoplasmas from the genital tract." @default.
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- W1336211 date "1999-01-01" @default.
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- W1336211 title "Mouse Model of Mycoplasma Genital Infections" @default.
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- W1336211 doi "https://doi.org/10.1016/b978-012775390-4/50188-3" @default.
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