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- W134051715 abstract "Cells are incubated continuously in bromodeoxyuridine (BrdUrd), which is incorporated into cells synthesizing DNA. At intervals, cells are harvested and nuclei are prepared and stained with a bis-benzimidazole, Hoechst 33258, and propidium iodide. In the flow cytometer, the dyes are excited by UV and blue and red fluorescences recorded. BrdUrd quenches the blue fluorescence of the Hoechst dye. The degree of quenching records the progress of the cell through S phase(s); the red (PI) fluorescence yields the cell cycle phases. By this means, the progress of cells around the cell cycle can be followed and the effects of cytotoxic drugs, radiation, and other treatments observed." @default.
- W134051715 created "2016-06-24" @default.
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- W134051715 date "2004-02-19" @default.
- W134051715 modified "2023-10-16" @default.
- W134051715 title "Cell-Cycle Analysis of Asynchronous Populations" @default.
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- W134051715 doi "https://doi.org/10.1385/1-59259-773-4:345" @default.
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