Matches in SemOpenAlex for { <https://semopenalex.org/work/W13444718> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W13444718 endingPage "16" @default.
- W13444718 startingPage "5" @default.
- W13444718 abstract "The review describes the history of creation and development of the microchip technology and its role in the human genome project in Russia. The emphasis is placed on the three-dimensional gel-based microchips developed at the Center of Biological Microchips headed by A.D. Mirzabekov since 1988. The gel-based chips of the last generation, IMAGE chips (Immobilized Micro Array of Gel Elements), have a number of advantages over the previous versions. The microchips are manufactured by photo-initiated copolymerization of gel components and immobilized molecules (DNA, proteins, and ligands). This ensures an even distribution of the immobilized probe throughout the microchip gel element with a high yield (about 50% for oligonucleotides). The use of methacrylamide as a main component of the polymerization mixture resulted in a substantial increase of gel porosity without affecting its mechanical strength and stability, which allowed one to work with the DNA fragments of up to 500 nt in length, as well as with rather large protein molecules. At present, the gel-based microchips are widely applied to address different problems. The generic microchips containing a complete set of possible hexanucleotides are used to reveal the DNA motifs binding with different proteins and to study the DNA-protein interactions. The oligonucleotide microchips are a cheap and reliable tool of diagnostics designed for mass application. Biochips have been developed for identification of the tuberculosis pathogen and its antibiotic-resistant forms; for diagnostics of orthopoxviruses, including the smallpox virus; for diagnostics of the anthrax pathogen; and for identification of chromosomal rearrangements in leukemia patients. The protein microchips can be adapted for further use in proteomics. Bacterial and yeast cells were also immobilized in the gel, maintaining their viability, which open a wide potential for creation biosensors on the basis of microchips." @default.
- W13444718 created "2016-06-24" @default.
- W13444718 creator A5001170211 @default.
- W13444718 creator A5065948781 @default.
- W13444718 creator A5076289086 @default.
- W13444718 creator A5076358271 @default.
- W13444718 creator A5077489462 @default.
- W13444718 creator A5078102596 @default.
- W13444718 creator A5087977511 @default.
- W13444718 creator A5089294554 @default.
- W13444718 creator A5090187826 @default.
- W13444718 date "2004-03-27" @default.
- W13444718 modified "2023-09-23" @default.
- W13444718 title "[Microchips based on three dimensional gel cells: history and perspective]." @default.
- W13444718 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15042831" @default.
- W13444718 hasPublicationYear "2004" @default.
- W13444718 type Work @default.
- W13444718 sameAs 13444718 @default.
- W13444718 citedByCount "1" @default.
- W13444718 crossrefType "journal-article" @default.
- W13444718 hasAuthorship W13444718A5001170211 @default.
- W13444718 hasAuthorship W13444718A5065948781 @default.
- W13444718 hasAuthorship W13444718A5076289086 @default.
- W13444718 hasAuthorship W13444718A5076358271 @default.
- W13444718 hasAuthorship W13444718A5077489462 @default.
- W13444718 hasAuthorship W13444718A5078102596 @default.
- W13444718 hasAuthorship W13444718A5087977511 @default.
- W13444718 hasAuthorship W13444718A5089294554 @default.
- W13444718 hasAuthorship W13444718A5090187826 @default.
- W13444718 hasConcept C104317684 @default.
- W13444718 hasConcept C129312508 @default.
- W13444718 hasConcept C141231307 @default.
- W13444718 hasConcept C171250308 @default.
- W13444718 hasConcept C185592680 @default.
- W13444718 hasConcept C192562407 @default.
- W13444718 hasConcept C21951064 @default.
- W13444718 hasConcept C552990157 @default.
- W13444718 hasConcept C55493867 @default.
- W13444718 hasConcept C70721500 @default.
- W13444718 hasConcept C86803240 @default.
- W13444718 hasConcept C87892846 @default.
- W13444718 hasConceptScore W13444718C104317684 @default.
- W13444718 hasConceptScore W13444718C129312508 @default.
- W13444718 hasConceptScore W13444718C141231307 @default.
- W13444718 hasConceptScore W13444718C171250308 @default.
- W13444718 hasConceptScore W13444718C185592680 @default.
- W13444718 hasConceptScore W13444718C192562407 @default.
- W13444718 hasConceptScore W13444718C21951064 @default.
- W13444718 hasConceptScore W13444718C552990157 @default.
- W13444718 hasConceptScore W13444718C55493867 @default.
- W13444718 hasConceptScore W13444718C70721500 @default.
- W13444718 hasConceptScore W13444718C86803240 @default.
- W13444718 hasConceptScore W13444718C87892846 @default.
- W13444718 hasIssue "1" @default.
- W13444718 hasLocation W134447181 @default.
- W13444718 hasOpenAccess W13444718 @default.
- W13444718 hasPrimaryLocation W134447181 @default.
- W13444718 hasRelatedWork W1938673075 @default.
- W13444718 hasRelatedWork W1972401981 @default.
- W13444718 hasRelatedWork W1989358260 @default.
- W13444718 hasRelatedWork W2140008189 @default.
- W13444718 hasRelatedWork W2245590541 @default.
- W13444718 hasRelatedWork W2461218155 @default.
- W13444718 hasRelatedWork W2724374374 @default.
- W13444718 hasRelatedWork W2771696728 @default.
- W13444718 hasRelatedWork W3108077828 @default.
- W13444718 hasRelatedWork W906914807 @default.
- W13444718 hasVolume "38" @default.
- W13444718 isParatext "false" @default.
- W13444718 isRetracted "false" @default.
- W13444718 magId "13444718" @default.
- W13444718 workType "article" @default.