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- W134529909 abstract "To investigate the gene transcription and expression related to virus infection,suppression subtractive hybridization technique was used to identify the up-regulated or down-regulated genes in the target cells(PAM) following PRRSV infection.In forward-subtraction,the cells infected by highly pathogenic porcine reproductive and respiratory syndrome virus(HP-PRRSV) HuN4 strain were taken as tester and non-infected as driver;In reverse-subtraction,the non-infected cells were taken as tester and infected as driver.The up-regulated and down-regulated genes were PCR amplified,ligated and transformed into E.coli cells to obtain differential transcription gene library.Nested PCR confirmed the high diversity of the library." @default.
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- W134529909 date "2009-01-01" @default.
- W134529909 modified "2023-09-24" @default.
- W134529909 title "Construction of subtraction library of porcine alveolar macrophages infected by highly pathogenic porcine reproductive and respiratory syndrome virus" @default.
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