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- W134617139 abstract "This chapter discusses monoclonal antibody production, which is an effective technique for fusing antibody-producing mouse spleen cells and immortalized mouse myeloma cells for isolation of the antibody-producing clones with amplification of the antibody product. Following dilution to single-cell aliquots, clones are grown and screened for antibody produced to a specific product. The method of hybridoma fusion allows for the production of highly specific antibody in quantity for further use. The time taken from first injection for the identification of positive clones is four months. The special equipment required for this method includes Coulter counter or other cell counter, cell-culture equipment, and 12-channel pipettor. In advance, inoculate mouse with desired antigen is required. Inoculation depends on the antigen used and its availability and purity. The inoculation can be with nonpure samples because the monoclonal antibody isolated will be directed against a single epitope. Pure antigen is needed for the screening assay." @default.
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- W134617139 date "1986-01-01" @default.
- W134617139 modified "2023-09-24" @default.
- W134617139 title "Monoclonal Antibody Production: Hybridoma Fusion" @default.
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- W134617139 doi "https://doi.org/10.1016/b978-0-444-01082-7.50093-x" @default.
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