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• W136136595 abstract "The emergence and re-emergence of transboundary animal diseases (TADs), e.g., foot-and-mouth disease, classical swine fever and the highly pathogenic avian influenza strongly indicate the need for the development of powerful and robust new diagnostic methods. The experiences of an OIE-Collaborating Centre and of two EU project consortia are summarised on the diagnostic application of gel-based PCR, general PCR systems, phylogeny, molecular epidemiology, real-time PCR (TaqMan, Molecular Beacons, Primer-Probe Energy Transfer), amplification without thermocycling (Invader), multiplex PCR, nucleic acid extraction and pipetting robotics, automation and quality control, including internal controls. By following the steps of OIE validation, the diagnostic assays are nationally and internationally standardised. The development of padlock probes and microarrays, as well as ultra rapid PCR and sequencing methods is further improving the arsenal of nucleic acid based molecular diagnosis. Further trends of diagnostic development are also mentioned, in order to combat TADs and other viral infections more effectively in the future." @default.
• W136136595 created "2016-06-24" @default.
• W136136595 creator A5067345562 @default.
• W136136595 date "2007-07-01" @default.
• W136136595 modified "2023-10-17" @default.
• W136136595 title "Molecular diagnosis of viral diseases, present trends and future aspects" @default.
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• W136136595 doi "https://doi.org/10.1016/j.vaccine.2006.11.068" @default.
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