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- W136280131 abstract "Scan RNAs (scnRNAs) are developmentally regulated siRNAs of ~26–32 nucleotides in length that are involved in programmed DNA elimination in Tetrahymena. scnRNAs are loaded onto the Piwi-related protein Twi1p and 2′-O-methylated at their 3′ termini. We describe two alternative strategies for analyzing the Twi1p-loaded scnRNAs: preparation of loaded scnRNAs by immuno-purification of the Twi1p–scnRNA complex and exclusion of non-methylated scnRNAs during cDNA library construction using periodate oxidation." @default.
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- W136280131 date "2013-10-07" @default.
- W136280131 modified "2023-10-16" @default.
- W136280131 title "Analysis of Piwi-Loaded Small RNAs in Terahymena" @default.
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- W136280131 doi "https://doi.org/10.1007/978-1-62703-694-8_17" @default.
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