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- W136987994 abstract "Considering the menace of transmission of bovine spongiform encephalopathy, feed components intended for cattle nutrition must be checked for the presence of bovine-derived materials. We have been using a method based on polymerase chain reaction for the identification of bovine-specific mitochondrial DNA sequences for this purpose. The specificity of the primers for polymerase chain reaction has been tested using samples of DNA of other vertebrate species, which may also be present in rendering plant products. The method allows the detection in concentrate mixtures of 0.125% of bovine-derived material. Bovine DNA at concentrations corresponding to less than 0.5% of bovine-derived material was detected in 3 of the 30 samples of concentrate mixtures collected from distributors' stores all over the Czech Republic. All 44 samples of fish meal collected from the same sources were free of bovine-derived material." @default.
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- W136987994 date "2001-01-01" @default.
- W136987994 modified "2023-09-26" @default.
- W136987994 title "Identification of Bovine-Specific DNA in Feedstuffs" @default.
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- W136987994 doi "https://doi.org/10.4315/0362-028x-64.1.117" @default.
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