Matches in SemOpenAlex for { <https://semopenalex.org/work/W13716952> ?p ?o ?g. }
- W13716952 abstract "Recently, artificial microRNA (miRNA)-mediated RNA interference (RNAi) systems have been developed as useful tools to study gene functions. We report an artificial miRNA precursor motif (AMPM) containing several restriction sites in the loop and in the extended stem structures, and generated a vector-based expression system for AMPM under the control of an RNA polymerase II promoter. The AMPM located in the intron or the exon of the selection marker gene mediated silencing of the reporter luciferase gene expression in HeLa cells. Northern blotting and colony formation assays revealed that the AMPM was efficiently and appropriately processed into mature miRNA. The AMPA system also down-regulated endogenous p53 and laminA/C proteins in stable transfectants. Moreover, clustered AMPMs separated by an appropriate spacer, targeting different sites within a single mRNA mediated gene silencing, even if the sequences of the target and the AMPM were partially complementary. This indicates the potential therapeutic utility of clustered AMPMs for highly-mutable targets, such as human immunodeficiency virus type 1." @default.
- W13716952 created "2016-06-24" @default.
- W13716952 creator A5007524794 @default.
- W13716952 creator A5036873842 @default.
- W13716952 creator A5076504407 @default.
- W13716952 date "2007-05-27" @default.
- W13716952 modified "2023-09-23" @default.
- W13716952 title "A novel expression system for artificial miRNAs containing no endogenous miRNA precursor sequences." @default.
- W13716952 cites W144423133 @default.
- W13716952 cites W1491989688 @default.
- W13716952 cites W1524036792 @default.
- W13716952 cites W1611419331 @default.
- W13716952 cites W1967633197 @default.
- W13716952 cites W1971643509 @default.
- W13716952 cites W1985974546 @default.
- W13716952 cites W1991313185 @default.
- W13716952 cites W1992941735 @default.
- W13716952 cites W1993883459 @default.
- W13716952 cites W2010791234 @default.
- W13716952 cites W2012258341 @default.
- W13716952 cites W2014064723 @default.
- W13716952 cites W2020833650 @default.
- W13716952 cites W2031423487 @default.
- W13716952 cites W2032945651 @default.
- W13716952 cites W2037067282 @default.
- W13716952 cites W2038783205 @default.
- W13716952 cites W2041594569 @default.
- W13716952 cites W2042673698 @default.
- W13716952 cites W2047404721 @default.
- W13716952 cites W2055835964 @default.
- W13716952 cites W2058978176 @default.
- W13716952 cites W2062290101 @default.
- W13716952 cites W2071596792 @default.
- W13716952 cites W2095125813 @default.
- W13716952 cites W2110029412 @default.
- W13716952 cites W2111436354 @default.
- W13716952 cites W2133763262 @default.
- W13716952 cites W2136148915 @default.
- W13716952 cites W2140091023 @default.
- W13716952 cites W2141157874 @default.
- W13716952 cites W2146642335 @default.
- W13716952 cites W2146900615 @default.
- W13716952 cites W2149485430 @default.
- W13716952 cites W2151922790 @default.
- W13716952 cites W2153478121 @default.
- W13716952 cites W2167557255 @default.
- W13716952 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2737215" @default.
- W13716952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19771221" @default.
- W13716952 hasPublicationYear "2007" @default.
- W13716952 type Work @default.
- W13716952 sameAs 13716952 @default.
- W13716952 citedByCount "1" @default.
- W13716952 countsByYear W137169522016 @default.
- W13716952 crossrefType "journal-article" @default.
- W13716952 hasAuthorship W13716952A5007524794 @default.
- W13716952 hasAuthorship W13716952A5036873842 @default.
- W13716952 hasAuthorship W13716952A5076504407 @default.
- W13716952 hasConcept C104317684 @default.
- W13716952 hasConcept C119056186 @default.
- W13716952 hasConcept C145059251 @default.
- W13716952 hasConcept C150194340 @default.
- W13716952 hasConcept C153911025 @default.
- W13716952 hasConcept C166703698 @default.
- W13716952 hasConcept C36823959 @default.
- W13716952 hasConcept C54355233 @default.
- W13716952 hasConcept C67705224 @default.
- W13716952 hasConcept C86803240 @default.
- W13716952 hasConcept C95444343 @default.
- W13716952 hasConceptScore W13716952C104317684 @default.
- W13716952 hasConceptScore W13716952C119056186 @default.
- W13716952 hasConceptScore W13716952C145059251 @default.
- W13716952 hasConceptScore W13716952C150194340 @default.
- W13716952 hasConceptScore W13716952C153911025 @default.
- W13716952 hasConceptScore W13716952C166703698 @default.
- W13716952 hasConceptScore W13716952C36823959 @default.
- W13716952 hasConceptScore W13716952C54355233 @default.
- W13716952 hasConceptScore W13716952C67705224 @default.
- W13716952 hasConceptScore W13716952C86803240 @default.
- W13716952 hasConceptScore W13716952C95444343 @default.
- W13716952 hasLocation W137169521 @default.
- W13716952 hasOpenAccess W13716952 @default.
- W13716952 hasPrimaryLocation W137169521 @default.
- W13716952 hasRelatedWork W1446487347 @default.
- W13716952 hasRelatedWork W1979390437 @default.
- W13716952 hasRelatedWork W1993883459 @default.
- W13716952 hasRelatedWork W1998494872 @default.
- W13716952 hasRelatedWork W2002053669 @default.
- W13716952 hasRelatedWork W2011674971 @default.
- W13716952 hasRelatedWork W2016125891 @default.
- W13716952 hasRelatedWork W2020326055 @default.
- W13716952 hasRelatedWork W2046440901 @default.
- W13716952 hasRelatedWork W2061566555 @default.
- W13716952 hasRelatedWork W2125841410 @default.
- W13716952 hasRelatedWork W2141004194 @default.
- W13716952 hasRelatedWork W2153175562 @default.
- W13716952 hasRelatedWork W2169575430 @default.
- W13716952 hasRelatedWork W2178626348 @default.
- W13716952 hasRelatedWork W2253477939 @default.
- W13716952 hasRelatedWork W3144080711 @default.
- W13716952 hasRelatedWork W6358032 @default.