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- W13718091 abstract "This chapter discusses microinjection of RNA and DNA into somatic cells. The transfer of molecules into eukaryotic cells by microinjection provides an efficient method to study the biological and biochemical activity and the fate of the molecules in their native environment, the living cell. The microinjection technique has almost no cell type restriction; moreover, suspension culture cells, for example, lymphocytes, oocytes, eggs, plant protoplasts, are accessible for microinjection. For this, the cells either are held by a capillary or are bound to a substrate by suitable linkers. Microinjection is by far the most efficient procedure. Only microinjection allows the transfer of a known number of test molecules either into the cytoplasm or into the nuclei of the recipient cell. Up to 100% of the recipient cells support expression of the transferred material, and stable transformed cell lines can be isolated with a frequency of 20–30 %." @default.
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- W13718091 date "1994-01-01" @default.
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- W13718091 title "Microinjection of RNA and DNA into Somatic Cells" @default.
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- W13718091 doi "https://doi.org/10.1016/b978-0-12-164717-9.50005-2" @default.
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