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• W13725583 abstract "Research Article15 May 1996free access Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisic acid biosynthesis and corresponding to the ABA locus of Arabidopsis thaliana. E. Marin E. Marin INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author L. Nussaume L. Nussaume INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author A. Quesada A. Quesada INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author M. Gonneau M. Gonneau INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author B. Sotta B. Sotta INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author P. Hugueney P. Hugueney INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author A. Frey A. Frey INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author A. Marion-Poll A. Marion-Poll INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author E. Marin E. Marin INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author L. Nussaume L. Nussaume INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author A. Quesada A. Quesada INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author M. Gonneau M. Gonneau INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author B. Sotta B. Sotta INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author P. Hugueney P. Hugueney INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author A. Frey A. Frey INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author A. Marion-Poll A. Marion-Poll INRA, Laboratorie de Biologie Cellulaire, France. Search for more papers by this author Author Information E. Marin1, L. Nussaume1, A. Quesada1, M. Gonneau1, B. Sotta1, P. Hugueney1, A. Frey1 and A. Marion-Poll1 1INRA, Laboratorie de Biologie Cellulaire, France. The EMBO Journal (1996)15:2331-2342https://doi.org/10.1002/j.1460-2075.1996.tb00589.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Abscisic acid (ABA) is a plant hormone which plays an important role in seed development and dormancy and in plant response to environmental stresses. An ABA-deficient mutant of Nicotiana plumbaginifolia, aba2, was isolated by transposon tagging using the maize Activator transposon. The aba2 mutant exhibits precocious seed germination and a severe wilty phenotype. The mutant is impaired in the first step of the ABA biosynthesis pathway, the zeaxanthin epoxidation reaction. ABA2 cDNA is able to complement N.plumbaginifolia aba2 and Arabidopsis thaliana aba mutations indicating that these mutants are homologous. ABA2 cDNA encodes a chloroplast-imported protein of 72.5 kDa, sharing similarities with different mono-oxigenases and oxidases of bacterial origin and having an ADP-binding fold and an FAD-binding domain. ABA2 protein, produced in Escherichia coli, exhibits in vitro zeaxanthin epoxidase activity. This is the first report of the isolation of a gene of the ABA biosynthetic pathway. The molecular identification of ABA2 opens the possibility to study the regulation of ABA biosynthesis and its cellular location. Previous ArticleNext Article Volume 15Issue 101 May 1996In this issue RelatedDetailsLoading ..." @default.
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• W13725583 title "Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisic acid biosynthesis and corresponding to the ABA locus of Arabidopsis thaliana." @default.
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