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- W137272445 abstract "Genomic amplification with transcript sequencing (GAWTS) (Stoflet et al., 1988; Sommer et al., 1990) is a generally applicable method for direct sequencing of PCR material. GAWTS is centered around the attachment of a phage promoter sequence (T7, SP6, or T3) to the 5′-end of one or both PCR primers. The phage promoter sequence allows the PCR product to be transcribed into RNA. Subsequently, the RNA is utilized as a single-stranded template for dideoxynucleotide sequencing with reverse transcriptase (Figure 18.1)." @default.
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- W137272445 date "1994-01-01" @default.
- W137272445 modified "2023-09-24" @default.
- W137272445 title "Phage Promoter-Based Methods for Sequencing and Screening for Mutations" @default.
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- W137272445 doi "https://doi.org/10.1007/978-1-4612-0257-8_18" @default.
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