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• W137276951 abstract "Research Article1 February 1992free access Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism. S. Usuda S. Usuda Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author T. Takemori T. Takemori Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author M. Matsuoka M. Matsuoka Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author T. Shirasawa T. Shirasawa Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author K. Yoshida K. Yoshida Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author A. Mori A. Mori Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author K. Ishizaka K. Ishizaka Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author H. Sakano H. Sakano Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author S. Usuda S. Usuda Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author T. Takemori T. Takemori Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author M. Matsuoka M. Matsuoka Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author T. Shirasawa T. Shirasawa Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author K. Yoshida K. Yoshida Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author A. Mori A. Mori Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author K. Ishizaka K. Ishizaka Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author H. Sakano H. Sakano Department of Molecular and Cell Biology, University of California, Berkeley 94720. Search for more papers by this author Author Information S. Usuda1, T. Takemori1, M. Matsuoka1, T. Shirasawa1, K. Yoshida1, A. Mori1, K. Ishizaka1 and H. Sakano1 1Department of Molecular and Cell Biology, University of California, Berkeley 94720. The EMBO Journal (1992)11:611-618https://doi.org/10.1002/j.1460-2075.1992.tb05093.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing ablts. This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline VH segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre-B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region. Previous ArticleNext Article Volume 11Issue 21 February 1992In this issue RelatedDetailsLoading ..." @default.
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• W137276951 title "Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism." @default.
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