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- W137377039 abstract "Publisher Summary This chapter describes the principles and practices of centrifugation techniques. There are three main types of centrifugal fractionation, namely, (1) differential pelleting (differential centrifugation); (2) rate-zonal density-gradient sedimentation; and (3) isopycnic density-gradient sedimentation. Of these techniques, differential pelleting is the method most commonly used for fractionating material according to size. In this method, the material to be fractionated is initially distributed uniformly throughout the sample solution, which is the sole occupant of the centrifuge tube. The efficiency of the fractionation of particles according to size and shape can be improved markedly by using rate-zonal centrifugation through a density gradient, although this does mean that the sample capacity of each tube is greatly reduced. This technique involves layering the sample on to the top of a liquid column that is stabilized by a gradient of an inert solute, commonly sucrose. The third method for separating particles is isopycnic sedimentation in a gradient whose maximum density exceeds that of the particles. This is an equilibrium technique in which particles are separated on the basis of their buoyant densities, independently of the time of centrifugation and of the size and shape of the particles, although these parameters do determine the rate at which equilibrium is reached and the width of the bands formed at equilibrium." @default.
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- W137377039 date "1978-01-01" @default.
- W137377039 modified "2023-09-30" @default.
- W137377039 title "Introduction: Principles and Practices of Centrifugation" @default.
- W137377039 doi "https://doi.org/10.1016/b978-0-408-70803-6.50005-4" @default.
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