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• W137643597 abstract "The development of zinc finger nucleases for targeted gene modification can benefit from rapid functional assays that directly quantify activity at the endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand break repair via non-homologous end joining. The assay is based on the ability of the Surveyor nuclease to selectively cleave distorted duplex DNA formed via cross-annealing of mutated and wild-type sequence." @default.
• W137643597 created "2016-06-24" @default.
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• W137643597 date "2010-01-01" @default.
• W137643597 modified "2023-10-12" @default.
• W137643597 title "A Rapid and General Assay for Monitoring Endogenous Gene Modification" @default.
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• W137643597 doi "https://doi.org/10.1007/978-1-60761-753-2_15" @default.
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