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- W13839711 abstract "This chapter discusses the characterization of the effector cell in an antibody-dependent cell-mediated cytotoxicity system. A study described in the chapter utilized herpes simplex virus (HSV) type-I-infected Chang liver cells coated with human anti-HSV antibody as targets and human mononuclear cell preparations as effectors. Human peripheral blood mononuclear cells (MC), after Ficoll/Hypaque separation, were further purified by a variety of techniques, including carbonyl iron depletion, plastic adherence, nylon wool adherence, and E rosette depletion, alone and in combination. Evaluation of membrane markers was done at all stages of the separation procedures. Plastic adherence routinely removed 80–90% of the latex positive monocytes from the MC preparations but did not lead to depletion of the ADCC activity, suggesting that the major effector cell was not a monocyte. Carbonyl iron depletion experiments also suggested that the effector cell was not a monocyte, though this technique is even more efficient in removing latex-positive cells." @default.
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- W13839711 date "1977-01-01" @default.
- W13839711 modified "2023-09-25" @default.
- W13839711 title "CHARACTERIZATION OF THE EFFECTOR CELL IN AN ANTIBODY DEPENDENT CELL MEDIATED CYTOTOXICITY SYSTEM" @default.
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- W13839711 doi "https://doi.org/10.1016/b978-0-12-458050-3.50082-5" @default.
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