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- W13839765 abstract "RNA interference (RNAi) is a mechanism by which the introduction of small interfering RNAs (siRNAs) into cultured cells causes degradation of the complementary mRNA. Applications of RNAi include gene function analysis, pathway analysis, and target validation. While RNAi experiments have become common practice in research labs, multiple factors can influence the extent of siRNA-induced knockdown (and thus biological outcome). A properly designed and selected siRNA sequence, siRNA modification format, choice of transfection reagent/technique, optimized protocols of siRNA in vitro delivery, and an appropriate and optimized readout are all critical for ensuring a successful experiment. In this chapter, we describe a typical in vitro siRNA experiment with optimization of transfection conditions and analysis of siRNA potency, i.e., mRNA knockdown with quantitative real-time PCR." @default.
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- W13839765 date "2011-01-01" @default.
- W13839765 modified "2023-09-23" @default.
- W13839765 title "Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR" @default.
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- W13839765 doi "https://doi.org/10.1007/978-1-61779-188-8_13" @default.
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