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- W138794865 abstract "To investigate the differences of recombinant vectors introducing ubiquitous expression and tissue-specific expression in Caenorhabditis elegans,the core promoter region of Act-1 gene and T07F10 gene,and the Poly(A) signal region of T07F10 gene were amplified from the genomic DNA of C. elegans using PCR,and SV40 early mRNA sequence and EGFP gene were amplified from eukaryotic vector pEGFP-N1,respectively. Taking the cloning vector pBluescript SK+ as backbone,four varied recombinant vectors were constructed,two of which,that is recombinant vectors pB-Pact-EGFP-T07F10T and pB-Pact-EGFP-SV40T,were expressed ubiquitously in C. elegans,regulated by the core promoter region of Act-1 gene,and the others,named as recombinant vectors pB-PT07F10-EGFP-T07F10T and pB-PT07F10-EGFP-SV40T,were expressed distinctively in specific tissues of C. elegans,regulated by the core promoter region of T07F10 gene. Further expression exploitation with these four recombinant vectors probably provided substantial information on different expression patterns using transgene technique in C. elegans." @default.
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- W138794865 date "2009-01-01" @default.
- W138794865 modified "2023-09-23" @default.
- W138794865 title "Construction of recombinant vectors introducing ubiquitous expression or tissue-specific expression in Caenorhabditis elegans." @default.
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