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- W138845303 abstract "This chapter focuses on the composition and function of centrosomes in vertebrates. The centrosome is the microtubule organizing center of animal cells. During cell division, the duplication and segregation of centrosomes dictate the bipolarity of the mitotic spindle and thus determine the plane of the cleavage furrow. The centrosome is composed of two centrioles tightly associated with a matrix, which consists of a complex thin filament network. In addition, two sets of appendages surround the mother centriole. The centrosome is an intriguing single copy organelle, the reproduction mechanism of which is still unknown. The function and the precise localization of the majority of these components represent a promising field of investigation. During the past decade, several methods for centrosome isolation have been described. Different starting materials, including cultured animal cells, animal tissues, Drosophila embryos, marine eggs, and fungi have been used. The chapter provides a brief overview of these methods and discusses the results and limitations of these approaches. The chapter also discusses the recent use of evolutionary conservation to identify essential centrosomal components and results from the past studies for future studies of this unique organelle." @default.
- W138845303 created "2016-06-24" @default.
- W138845303 creator A5002753836 @default.
- W138845303 creator A5052665492 @default.
- W138845303 date "1998-01-01" @default.
- W138845303 modified "2023-10-15" @default.
- W138845303 title "Chapter 2 Studying the Composition and Function of Centrosomes in Vertebrates" @default.
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- W138845303 doi "https://doi.org/10.1016/s0091-679x(08)61973-1" @default.
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