FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W13939407> ?p ?o ?g. }

• W13939407 abstract "Abiotic stresses, such as extreme temperatures, soil salinity, or water deficit, are one of the major limiting factors of crop productivity worldwide. Examination of molecular and genetic mechanisms of abiotic stress tolerance in plants is of great interest to plant biologists. Calcium-dependent protein kinases (CDPKs), which are the most important Ca sensors in plants, are known to play one of the key roles in plant adaptation to abiotic stress. CDPK is a multigene family of enzymes. Analysis of CDPK gene expression under various abiotic stress conditions would help identify those CDPKs that might play important roles in plant adaptation to abiotic stress. We focused on studying CDPK gene expression under osmotic, water deficit, and temperature stress conditions in a wild-growing grapevine Vitis amurensis Rurp., which is native to the Russian Far East and is known to possess high adaptive potential and high level of resistance against adverse environmental conditions. Healthy V. amurensis cuttings (excised young stems with one healthy leaf) were used for the treatments. For the non-stress treatment, we placed the cuttings in distilled water for 12 h at room temperature. For the water-deficit stress, detached cuttings were laid on a paper towel for 12 h at room temperature. For osmotic stress treatments, the cuttings were placed in 0.4 М NaCl and 0.4 М mannitol solutions for 12 h at room temperature. To examine temperature stress tolerance, the V. amurensis cuttings were placed in a growth chamber at +10C and +37C for 12 h. The total expression of VaCDPK genes was examined by semiquantitative RT-PCR with degenerate primers designed to the CDPK kinase domain. The total level of CDPK gene expression increased under salt and decreased under low temperature stress conditions. We sequenced 300 clones of the amplified part of different CDPK transcripts obtained from the analyzed cDNA probes. Analysis of the cDNA sequences identified 8 different CDPK genes (VaCDPK1a, 1e, 1d, 2a, 3a, 3b, 3c, 3d). We sequenced full cDNA sequences of the genes and analyzed their expression levels by real-time PCR and FAPP method, which has been recently developed by our research group. The prevalent CDPK transcript was VaCDPK3a under both non-stress and abiotic stress conditions. Under high-salt conditions, VaCDPK1d, 1e, 3b, and 3d transcripts were up-regulated. Under high mannitol conditions, expression of VaCPK1e and 3b was up-regulated, while expression of VaCDPK1d, 3c, and 3d was only slightly induced. Under water-deficit, expression of only VaCDPK3b and 3c genes was induced. Cold stress induced expression of VaCDPK2a and 3d genes; while hot stress induced expression of VaCDPK1a, 1d, 1e, 2a, 3a, and 3c genes. Taken together, the data show that the VaCDPK genes are transcriptionally regulated by osmotic, water-deficit, and temperature stresses. The differential expression of the VaCDPK genes during osmotic, water-deficit, and temperature stresses is suggestive of their involvement in the underlying signal transduction pathways." @default.
• W13939407 created "2016-06-24" @default.
• W13939407 creator A5000083171 @default.
• W13939407 date "2012-01-01" @default.
• W13939407 modified "2023-09-24" @default.
• W13939407 title "Expression of calcium-dependent protein kinase (CDPK) genes in Vitis amurensis under abiotic stress conditions" @default.
• W13939407 hasPublicationYear "2012" @default.
• W13939407 type Work @default.
• W13939407 sameAs 13939407 @default.
• W13939407 citedByCount "0" @default.
• W13939407 crossrefType "journal-article" @default.
• W13939407 hasAuthorship W13939407A5000083171 @default.
• W13939407 hasConcept C104317684 @default.
• W13939407 hasConcept C129513315 @default.
• W13939407 hasConcept C132215390 @default.
• W13939407 hasConcept C139807058 @default.
• W13939407 hasConcept C144027150 @default.
• W13939407 hasConcept C168529131 @default.
• W13939407 hasConcept C169760540 @default.
• W13939407 hasConcept C187528503 @default.
• W13939407 hasConcept C18903297 @default.
• W13939407 hasConcept C54355233 @default.
• W13939407 hasConcept C59822182 @default.
• W13939407 hasConcept C86803240 @default.
• W13939407 hasConcept C89519541 @default.
• W13939407 hasConceptScore W13939407C104317684 @default.
• W13939407 hasConceptScore W13939407C129513315 @default.
• W13939407 hasConceptScore W13939407C132215390 @default.
• W13939407 hasConceptScore W13939407C139807058 @default.
• W13939407 hasConceptScore W13939407C144027150 @default.
• W13939407 hasConceptScore W13939407C168529131 @default.
• W13939407 hasConceptScore W13939407C169760540 @default.
• W13939407 hasConceptScore W13939407C187528503 @default.
• W13939407 hasConceptScore W13939407C18903297 @default.
• W13939407 hasConceptScore W13939407C54355233 @default.
• W13939407 hasConceptScore W13939407C59822182 @default.
• W13939407 hasConceptScore W13939407C86803240 @default.
• W13939407 hasConceptScore W13939407C89519541 @default.
• W13939407 hasIssue "3" @default.
• W13939407 hasLocation W139394071 @default.
• W13939407 hasOpenAccess W13939407 @default.
• W13939407 hasPrimaryLocation W139394071 @default.
• W13939407 hasRelatedWork W1987876778 @default.
• W13939407 hasRelatedWork W2003734590 @default.
• W13939407 hasRelatedWork W2072929992 @default.
• W13939407 hasRelatedWork W2144390643 @default.
• W13939407 hasRelatedWork W2151459111 @default.
• W13939407 hasRelatedWork W2216337140 @default.
• W13939407 hasRelatedWork W2473817963 @default.
• W13939407 hasRelatedWork W2618898269 @default.
• W13939407 hasRelatedWork W2727085439 @default.
• W13939407 hasRelatedWork W2890974615 @default.
• W13939407 hasRelatedWork W2912503342 @default.
• W13939407 hasRelatedWork W2942415617 @default.
• W13939407 hasRelatedWork W2945454221 @default.
• W13939407 hasRelatedWork W2951160025 @default.
• W13939407 hasRelatedWork W2982227749 @default.
• W13939407 hasRelatedWork W3046866925 @default.
• W13939407 hasRelatedWork W3093598551 @default.
• W13939407 hasRelatedWork W3135312938 @default.
• W13939407 hasRelatedWork W3145456062 @default.
• W13939407 hasRelatedWork W926719206 @default.
• W13939407 hasVolume "8" @default.
• W13939407 isParatext "false" @default.
• W13939407 isRetracted "false" @default.
• W13939407 magId "13939407" @default.
• W13939407 workType "article" @default.