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- W13965349 abstract "To simplify the procedures of isolation of rat Sertoli cells and increase the yield of Sertoli cells cultured.Decapsulated rat testis from 16-22-day-old SD rats were digested by sequential two-step digestion using 0.25% pancreatin, 0.05% collagenase in order, and then the cell suspension was cultured in incubator under the condition of 37 degrees C and 5% CO2 for 48 h. The yields were observed and Fas Ligand (Fas-L) was tested by immunohistochemical method.By the use of this modified method, the yielded sertoli cells accounted for 90% of all the cells harvested. High level expression of Fas-L was detected.This modified method increases the yield of Sertoli cells and the procedures of isolation and culture are greatly simplified." @default.
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- W13965349 date "2003-10-01" @default.
- W13965349 modified "2023-09-23" @default.
- W13965349 title "[The modified isolation and culture method of rat Sertoli cells]." @default.
- W13965349 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14619597" @default.
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