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• W139670830 abstract "A protein (Mr 24 K) that co-purifies with porcine skin lysyl oxidase (Mr 34 K) has been isolated and characterised. Five variants of the 24 K protein were identified by Mono Q ion-exchange FPLC, as were four variants of lysyl oxidase. Amino acid analysis and partial sequencing revealed near identity of a 36-residue CNBr peptide from porcine skin lysyl oxidase to corresponding regions of the putative lysyl oxidase precursor derived from rat and human cDNA. The 24 K protein was found to be unrelated to lysyl oxidase, but comparison with a protein sequence database showed it to be the same as a recently described protein from bovine skin that is associated with dermatan sulphate proteoglycans. The 24 K protein is relatively rich in tyrosine, and isoelectric focussing shows it to be acidic, with pI's in the range 4.1 to 4.4. In view of these properties, we propose the name TRAMP (Tyrosine Rich Acidic Matrix Protein) to identify this protein. Though TRAMP appears not to be glycosylated, several experiments indicate the presence of sulphotyrosine residues. When assayed using an elastin substrate, the activity of lysyl oxidase is unaffected by TRAMP." @default.
• W139670830 created "2016-06-24" @default.
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• W139670830 date "1993-05-01" @default.
• W139670830 modified "2023-09-27" @default.
• W139670830 title "TRAMP (Tyrosine Rich Acidic Matrix Protein), a Protein that Co-purifies with Lysyl Oxidase from Porcine Skin" @default.
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• W139670830 doi "https://doi.org/10.1016/s0934-8832(11)80009-0" @default.
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