Matches in SemOpenAlex for { <https://semopenalex.org/work/W139726621> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W139726621 abstract "A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola’s Blue (MB) has been investigated as the base transducer for a glutamate biosensor. The sandwich biosensor was fabricated by firstly depositing a chitosan (CHIT) layer onto the surface of the transducer (MB-SPCE), followed by glutamate dehydrogenase (GLDH): this device is designated GLDH-CHIT-MB-SPCE. NAD+ was added to buffer solutions prior to the measurement of glutamate. This biosensor was used in conjunction with amperometry in stirred solution at an applied potential of +0.1 V (vs. Ag/AgCl). Optimum conditions for the analysis of glutamate were found to be as follows: temperature, 35°C; buffer, pH 7; ionic strength, 75 mM; NAD+, 4 mM; CHIT 0.05% in 0.05 M HCl; GLDH, 30 U. The linear range of the biosensor was found to be 12.5 µM to 150 µM, the calculated limit of detection (based on three times signal to noise ratio) was 1.5 µM and the sensitivity was 0.44 nA/µM. The proposed biosensor was used to measure glutamate in serum before and after fortification with glutamate. The endogenous concentration of glutamate was found to be 1.68 mM and the coefficient of variation (CV) was 4.1%. The serum was then fortified with 2 mM of glutamate, and the resulting mean recovery was 96% with a CV of 3.3% (n = 6). An unfiltered beef OXO cube was analysed for monosodium glutamate (MSG) content. The endogenous content of MSG was 124.80 mg/g with a CV of 8.06%. The OXO cube solution was fortified with 0.935g (100 mM) of glutamate, the resulting mean recovery was 91% with a CV of 6.40 %." @default.
- W139726621 created "2016-06-24" @default.
- W139726621 creator A5015559660 @default.
- W139726621 creator A5041875789 @default.
- W139726621 creator A5057234642 @default.
- W139726621 creator A5081092479 @default.
- W139726621 date "2014-08-31" @default.
- W139726621 modified "2023-09-26" @default.
- W139726621 title "Development of a disposable screen-printed amperometric biosensor based on glutamate dehydrogenase, for the determination of glutamate in clinical and food applications" @default.
- W139726621 cites W1495445155 @default.
- W139726621 hasPublicationYear "2014" @default.
- W139726621 type Work @default.
- W139726621 sameAs 139726621 @default.
- W139726621 citedByCount "0" @default.
- W139726621 crossrefType "journal-article" @default.
- W139726621 hasAuthorship W139726621A5015559660 @default.
- W139726621 hasAuthorship W139726621A5041875789 @default.
- W139726621 hasAuthorship W139726621A5057234642 @default.
- W139726621 hasAuthorship W139726621A5081092479 @default.
- W139726621 hasConcept C113196181 @default.
- W139726621 hasConcept C119128265 @default.
- W139726621 hasConcept C139369640 @default.
- W139726621 hasConcept C13965031 @default.
- W139726621 hasConcept C147789679 @default.
- W139726621 hasConcept C160756335 @default.
- W139726621 hasConcept C170493617 @default.
- W139726621 hasConcept C17525397 @default.
- W139726621 hasConcept C178790620 @default.
- W139726621 hasConcept C184651966 @default.
- W139726621 hasConcept C185592680 @default.
- W139726621 hasConcept C187428577 @default.
- W139726621 hasConcept C2777202666 @default.
- W139726621 hasConcept C2778117296 @default.
- W139726621 hasConcept C43617362 @default.
- W139726621 hasConcept C52859227 @default.
- W139726621 hasConcept C55493867 @default.
- W139726621 hasConcept C61174792 @default.
- W139726621 hasConceptScore W139726621C113196181 @default.
- W139726621 hasConceptScore W139726621C119128265 @default.
- W139726621 hasConceptScore W139726621C139369640 @default.
- W139726621 hasConceptScore W139726621C13965031 @default.
- W139726621 hasConceptScore W139726621C147789679 @default.
- W139726621 hasConceptScore W139726621C160756335 @default.
- W139726621 hasConceptScore W139726621C170493617 @default.
- W139726621 hasConceptScore W139726621C17525397 @default.
- W139726621 hasConceptScore W139726621C178790620 @default.
- W139726621 hasConceptScore W139726621C184651966 @default.
- W139726621 hasConceptScore W139726621C185592680 @default.
- W139726621 hasConceptScore W139726621C187428577 @default.
- W139726621 hasConceptScore W139726621C2777202666 @default.
- W139726621 hasConceptScore W139726621C2778117296 @default.
- W139726621 hasConceptScore W139726621C43617362 @default.
- W139726621 hasConceptScore W139726621C52859227 @default.
- W139726621 hasConceptScore W139726621C55493867 @default.
- W139726621 hasConceptScore W139726621C61174792 @default.
- W139726621 hasIssue "4" @default.
- W139726621 hasLocation W1397266211 @default.
- W139726621 hasOpenAccess W139726621 @default.
- W139726621 hasPrimaryLocation W1397266211 @default.
- W139726621 hasRelatedWork W2003076695 @default.
- W139726621 hasRelatedWork W2011332648 @default.
- W139726621 hasRelatedWork W2043335115 @default.
- W139726621 hasRelatedWork W2057776760 @default.
- W139726621 hasRelatedWork W2059056551 @default.
- W139726621 hasRelatedWork W2091312425 @default.
- W139726621 hasRelatedWork W2092065393 @default.
- W139726621 hasRelatedWork W2095469984 @default.
- W139726621 hasRelatedWork W2099635401 @default.
- W139726621 hasRelatedWork W2255262896 @default.
- W139726621 hasRelatedWork W2344664429 @default.
- W139726621 hasRelatedWork W2404983683 @default.
- W139726621 hasRelatedWork W2413101387 @default.
- W139726621 hasRelatedWork W2416062034 @default.
- W139726621 hasRelatedWork W2418033208 @default.
- W139726621 hasRelatedWork W2422870969 @default.
- W139726621 hasRelatedWork W2460331595 @default.
- W139726621 hasRelatedWork W3031056282 @default.
- W139726621 hasRelatedWork W304136458 @default.
- W139726621 hasRelatedWork W3201231775 @default.
- W139726621 hasVolume "6" @default.
- W139726621 isParatext "false" @default.
- W139726621 isRetracted "false" @default.
- W139726621 magId "139726621" @default.
- W139726621 workType "article" @default.