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- W139817667 abstract "There are a variety of macromolecular systems exhibiting structural and dynamic heterogeneity, in which expected NMR signals are not always fully visible by a single set of NMR measurements. The former includes hydrogels of synthetic polymers, polysaccharides, and aggregated proteins, the latter of which can be further converted to amyloids or prions. They are very important as materials for biomedical or industrial applications, a variety of biological functions including host-defence systems, components of biological tissues, or key substances leading to several serious diseases. In addition, membrane proteins are typical dynamically heterogeneous systems which give rise to suppressed peaks depending upon local protein dynamics. In particular, it is emphasized that the presence of such suppressed peaks affords an invaluable means to determine their dynamic information. In this chapter, we first briefly review several parameters to be able to analyse their dynamic features. Then, we discuss two types of heterogeneous systems in more detail: structurally and/or dynamically heterogeneous systems. NMR studies on synthetic and polysaccharide gels and protein fibrils are reviewed with emphasis on revealing their secondary structures and dynamic aspects. Dynamic aspects of membrane proteins are also discussed in relation to their biological function. It turned out that the combined use of a solution (and or solution-like) state and a solid-state NMR technique are essential to be able to fully observe signals from portions of different mobility. We emphasize that recording spectra based on dipolar decoupled (or the direct detection) magic angle spinning (DDMAS) approach is essential to be able to reveal the more flexible portions of these systems, because the conventional solid-state NMR, using cross-polarization magic angle spinning (CPMAS) alone, is not sufficient for detecting signals from the solid-like portions." @default.
- W139817667 created "2016-06-24" @default.
- W139817667 creator A5031126871 @default.
- W139817667 date "2011-01-01" @default.
- W139817667 modified "2023-10-13" @default.
- W139817667 title "NMR Studies on Structurally or Dynamically Heterogeneous Systems" @default.
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