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- W140083588 abstract "ABSTRACT The studies on cryopreserved arterial allografts have been focused on cooling methods, pre-treatment, cryoprotectant agents, and preservation temperature. But recently, several studies have reported that thawing methods also play an important role in the occurrence of macroscopic and microscopic cracks. This study was designed to investigate the expression of apoptosis after thawing, using a rabbit model to clarify the effect of thawing methods on cryopreserved arteries. Segments of the rabbit aorta were obtained and divided into 3 groups (n=60) according to whether the specimens were fresh (control, n=20), cryopreserved and rapidly thawed (RT) at 37°C (n=20), or cryopreserved and subjected to controlled, automated slow thawing (ST) (n=20). Cell damage was established using the TUNEL method and the morphological and microstructural changes were also evaluated. In the group that was rapidly thawed, the expression of TUNEL (+) cells increased significantly more than in the slowly thawed group. In addition, the endothelial denudation, microvesicles and edema were significant in the rapidly thawed group compared with the microstructural changes in the slowly thawed group. Our study suggests that the rapidly thawing method may be the major cause of cellular damage and delayed rupture in cryopreserved arterial allografts. The expression of TUNEL (+) cells and structural changes were significantly lower in the slowly thawed group, which might have contributed to the improvement of graft failure after transplantation." @default.
- W140083588 created "2016-06-24" @default.
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- W140083588 date "2005-01-01" @default.
- W140083588 modified "2023-09-27" @default.
- W140083588 title "COMPARISON OF DIFFERENT THAWING METHODS ON CRYOPRESERVED RABBIT AORTA" @default.
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- W140083588 doi "https://doi.org/10.1533/9781845690779.2.123" @default.
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