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- W1408833436 abstract "The chapter discusses the methods and applications of explant culture of human colon. The specimen for the study is obtained from nontumorous human colonic tissues of patients with and without cancer of the colon at the time of either surgery or “immediate” autopsy. Determination of viability of cultured tissues was done based on morphology, immunological markers, and biological markers. The explants are maintained up to 14 days with near normal morphology after, which a decrease in both the number of intestinal glands and the number of mucus-producing cells was observed. One of the major problems in culturing human colon is the diffusion of nutrients to the glands. The phenomenon of encircling of the explant with epithelial cells is observed in cultured human bladder and in human bronchus. Explant cultures of human colon have been used in the study of biochemical parameters in both normal and diseased colonic tissues and for studies on colon carcinogenesis." @default.
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- W1408833436 date "1980-01-01" @default.
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- W1408833436 title "Chapter 19 Explant Culture of Human Colon" @default.
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- W1408833436 doi "https://doi.org/10.1016/s0091-679x(08)60694-9" @default.
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