FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W14103738> ?p ?o ?g. }

• W14103738 endingPage "682" @default.
• W14103738 startingPage "675" @default.
• W14103738 abstract "The uptake and metabolism of radiolabelled retinol was studied in cultivated human skin cells. Normal epidermal keratinocytes in primary culture were able to incorporate unbound [11,12-3H]all-trans-retinol from the growth medium and transform it into 3,4-didehydroretinol (dehydroretinol) in a dose- and time-dependent manner. A total of 23% of the radioactive label became cell-associated during a 48-h incubation period when added at 7 nM to differentiated keratinocytes submerged in serum-containing, high-calcium (1.56 mM) culture medium. At that time point, 25-30% of cell-bound radioactive retinol had been converted into dehydroretinol, with no labelled retinal, dehydroretinal, retinoic acid or dehydroretinoic acid being detected in cells or medium. Thus dehydroretinol, which occurs physiologically in mammalian skin tissue in vivo, was identified as the predominant neutral retinol metabolite in cultured keratinocytes using h.p.l.c. and anhydro-derivatization procedures. At least 94% of the product, along with its precursor, was present in the cells in esterified form, with no traces of the compound being secreted into the cell environment. The rate of formation of dehydroretinol from its precursor was significantly lower in keratinocytes grown in serum-free, low-calcium (0.09 mM) culture medium, and in medium pre-incubated with excess unlabelled substrate. Furthermore, the application of 13-cis-retinoic acid (isotretinoin), a therapeutic retinoid drug known to markedly reduce dehydroretinol levels in human skin, blocked the biosynthesis of this metabolite in cultured keratinocytes. The 3,4-dehydrogenation pathway observed in this study could not be shown to operate to any significant extent in cultures of human epidermal melanocytes or dermal fibroblasts, supporting the hypothesis that keratinocytes represent the principal cell type involved in dehydroretinol formation from retinol in human skin." @default.
• W14103738 created "2016-06-24" @default.
• W14103738 creator A5060979655 @default.
• W14103738 creator A5071264647 @default.
• W14103738 creator A5086273885 @default.
• W14103738 creator A5090641181 @default.
• W14103738 date "1993-08-01" @default.
• W14103738 modified "2023-09-26" @default.
• W14103738 title "Biosynthesis of 3,4-didehydroretinol from retinol by human skin keratinocytes in culture" @default.
• W14103738 cites W1503735972 @default.
• W14103738 cites W1968188698 @default.
• W14103738 cites W1974454826 @default.
• W14103738 cites W1977824007 @default.
• W14103738 cites W1981632777 @default.
• W14103738 cites W1984509210 @default.
• W14103738 cites W1999960701 @default.
• W14103738 cites W2003008249 @default.
• W14103738 cites W2010579819 @default.
• W14103738 cites W2012761399 @default.
• W14103738 cites W2045597040 @default.
• W14103738 cites W2049791242 @default.
• W14103738 cites W2050672727 @default.
• W14103738 cites W2052144075 @default.
• W14103738 cites W2064418777 @default.
• W14103738 cites W2072233737 @default.
• W14103738 cites W2077406309 @default.
• W14103738 cites W2082376642 @default.
• W14103738 cites W2082897217 @default.
• W14103738 cites W2083436346 @default.
• W14103738 cites W2085552622 @default.
• W14103738 cites W2088438981 @default.
• W14103738 cites W2117847829 @default.
• W14103738 cites W2150962602 @default.
• W14103738 cites W2155544653 @default.
• W14103738 cites W2161399727 @default.
• W14103738 cites W2186930632 @default.
• W14103738 cites W22292592 @default.
• W14103738 cites W2261453368 @default.
• W14103738 cites W2417082560 @default.
• W14103738 doi "https://doi.org/10.1042/bj2930675" @default.
• W14103738 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1134419" @default.
• W14103738 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8352734" @default.
• W14103738 hasPublicationYear "1993" @default.
• W14103738 type Work @default.
• W14103738 sameAs 14103738 @default.
• W14103738 citedByCount "30" @default.
• W14103738 countsByYear W141037382012 @default.
• W14103738 countsByYear W141037382016 @default.
• W14103738 countsByYear W141037382017 @default.
• W14103738 countsByYear W141037382018 @default.
• W14103738 countsByYear W141037382021 @default.
• W14103738 countsByYear W141037382022 @default.
• W14103738 crossrefType "journal-article" @default.
• W14103738 hasAuthorship W14103738A5060979655 @default.
• W14103738 hasAuthorship W14103738A5071264647 @default.
• W14103738 hasAuthorship W14103738A5086273885 @default.
• W14103738 hasAuthorship W14103738A5090641181 @default.
• W14103738 hasBestOaLocation W141037382 @default.
• W14103738 hasConcept C104317684 @default.
• W14103738 hasConcept C153911025 @default.
• W14103738 hasConcept C181199279 @default.
• W14103738 hasConcept C185592680 @default.
• W14103738 hasConcept C202751555 @default.
• W14103738 hasConcept C25642318 @default.
• W14103738 hasConcept C2775954498 @default.
• W14103738 hasConcept C2776940978 @default.
• W14103738 hasConcept C2777074287 @default.
• W14103738 hasConcept C2777459323 @default.
• W14103738 hasConcept C2777477808 @default.
• W14103738 hasConcept C2779573558 @default.
• W14103738 hasConcept C2781121885 @default.
• W14103738 hasConcept C54355233 @default.
• W14103738 hasConcept C553450214 @default.
• W14103738 hasConcept C55493867 @default.
• W14103738 hasConcept C62231903 @default.
• W14103738 hasConcept C81885089 @default.
• W14103738 hasConcept C86803240 @default.
• W14103738 hasConcept C90703135 @default.
• W14103738 hasConceptScore W14103738C104317684 @default.
• W14103738 hasConceptScore W14103738C153911025 @default.
• W14103738 hasConceptScore W14103738C181199279 @default.
• W14103738 hasConceptScore W14103738C185592680 @default.
• W14103738 hasConceptScore W14103738C202751555 @default.
• W14103738 hasConceptScore W14103738C25642318 @default.
• W14103738 hasConceptScore W14103738C2775954498 @default.
• W14103738 hasConceptScore W14103738C2776940978 @default.
• W14103738 hasConceptScore W14103738C2777074287 @default.
• W14103738 hasConceptScore W14103738C2777459323 @default.
• W14103738 hasConceptScore W14103738C2777477808 @default.
• W14103738 hasConceptScore W14103738C2779573558 @default.
• W14103738 hasConceptScore W14103738C2781121885 @default.
• W14103738 hasConceptScore W14103738C54355233 @default.
• W14103738 hasConceptScore W14103738C553450214 @default.
• W14103738 hasConceptScore W14103738C55493867 @default.
• W14103738 hasConceptScore W14103738C62231903 @default.
• W14103738 hasConceptScore W14103738C81885089 @default.
• W14103738 hasConceptScore W14103738C86803240 @default.
• W14103738 hasConceptScore W14103738C90703135 @default.

• next