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- W1413403744 abstract "This chapter discusses the development of the methods for separating inner and outer membranes of mitochondria. Over 100 enzymes have been recognized as sites of localization within the mitochondrial structural framework from a variety of cell types and tissues. Changes in the methods of mitochondrial membrane separation include the use of proteases, various concentrations of digitonin, alterations in speeds of centrifugation or in gradient composition, the addition of ionophores to increase swelling, the inclusion of polyvinyl sulfate in the separation medium, and variations in time of incubation with digitonin. Although there is a general agreement to the localization of marker enzymes and of those shared or in common with mitochondria from different sources, some disagreement still exists. These differences are related to variations in the details of the methodology applied by different laboratories, to the possible heterogeneity of the inner membrane, the existence of contact sites between the two membranes, difficulties in distinguishing between extrinsic membrane-associated proteins and adsorbed enzyme activities, the reversible release and rebinding of enzymes from the inner mitochondrial compartment, and transmembrane interaction." @default.
- W1413403744 created "2016-06-24" @default.
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- W1413403744 date "1979-01-01" @default.
- W1413403744 modified "2023-10-13" @default.
- W1413403744 title "[10] Survey and update of outer and inner mitochondrial membrane separation" @default.
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- W1413403744 doi "https://doi.org/10.1016/0076-6879(79)55012-5" @default.
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