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- W1413551270 abstract "Hydrazinolysis is an effective technique for the release of unreduced O-linked oligosaccharides from glycoproteins. The mechanism leading to the release of O-linked oligosaccharides has not yet been investigated, although β elimination is suspected. Reaction conditions under which N- and O-linked oligosaccharides can be released from glycoproteins have been established empirically after investigation of the release of such oligosaccharides from a range of glycoprotein standards, whose glycosylation characteristics had been previously well defined. Despite the structural diversity of oligosaccharides and protein moieties associated with these standards, similar reaction conditions are found to allow essentially complete release of unreduced N- and O-linked oligosaccharides. Selective release of O-linked oligosaccharides with small release of N-linked oligosaccharides can be achieved with mild hydrazinolysis reaction conditions. Hydrazinolysis can therefore be used in three different ways, namely—to release all (N- and O-linked) oligosaccharides from a glycoprotein, to release selectively just O-linked oligosaccharides, and to release sequentially first O-linked, and then N-linked oligosaccharides." @default.
- W1413551270 created "2016-06-24" @default.
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- W1413551270 date "1994-01-01" @default.
- W1413551270 modified "2023-10-14" @default.
- W1413551270 title "[5] Release of oligosaccharides from glycoproteins by hydrazinolysis" @default.
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- W1413551270 doi "https://doi.org/10.1016/0076-6879(94)30007-0" @default.
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