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- W1414804906 abstract "Publisher Summary This chapter presents the assay procedure and properties of streptomycin-6-P phosphohydrolase. In assay, dephosphorylation of [3'α- 3 H] dihydrostreptomycin-6-P is followed by paper chromatography. This assay can be used for both crude extracts and partially purified preparations. This assay should be employed at intervals, during purification procedures that utilize less specific assays, to be certain that the desired phosphatase is being isolated. In second method of assay, streptomycin-6-P phosphatase (N) catalyzes transfer of phosphate from dihydrostreptomycin-6-P or p-nitrophenyl-P to amino alcohols such as Tris, streptamine, or 2-deoxystreptamine; esterification occurs adjacent to the basic group. In the first step of this 2-step enzymic assay, a solution containing streptomycin-6-P phosphatase catalyzes transfer of phosphate from p-nitrophenyl-P to streptamine or 2-deoxystreptamine. In the second step, EDTA is added to inhibit further phosphatase action, and the streptamine-P isomer is transamidinated in the presence of L-[guanidino- 14 C] arginine and a dialyzed extract containing inosamine-P amidinotransferase. The labeled product is separated by paper chromatography and counted." @default.
- W1414804906 created "2016-06-24" @default.
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- W1414804906 date "1975-01-01" @default.
- W1414804906 modified "2023-09-27" @default.
- W1414804906 title "[28] Streptomycin-6-P phosphohydrolase" @default.
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- W1414804906 doi "https://doi.org/10.1016/0076-6879(75)43104-4" @default.
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