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- W1415713615 abstract "Publisher Summary This chapter discusses Dowex 1 chromatography of oligonucleotides. The chapter describes successful separation of oligonucleotides in the ribonuclease digests using Dowex 1 chromatography. The low degree of cross-linking (2% cross-linking Dowex 1, 200-400 mesh) enables in equilibrating the larger oligonucleotides with the active groups of the resin, and produces the successful separation of oligonucleotides. Dowex 1 has the merit that makes it possible to separate not only the different-sized oligonucleotides, but also the oligonucleotides of the same size into single kinds of oligonucleotides in a single chromatography, although some of the components are superimposed on each other. Dowex 1 chromatography is useful for the structural study of nucleic acids, or for the preparation of single oligonucleotides from a mixture, and other chromatographic methods, such as the ion-exchange column chromatography using urea, or the mapping procedure for oligonucleotides on a filter paper with electrophoresis and chromatography." @default.
- W1415713615 created "2016-06-24" @default.
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- W1415713615 date "1967-01-01" @default.
- W1415713615 modified "2023-10-17" @default.
- W1415713615 title "[45] Dowex 1 chromatography of oligonucleotides" @default.
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- W1415713615 doi "https://doi.org/10.1016/s0076-6879(67)12054-5" @default.
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