Matches in SemOpenAlex for { <https://semopenalex.org/work/W1417509926> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1417509926 endingPage "4" @default.
- W1417509926 startingPage "1" @default.
- W1417509926 abstract "Objective To obtain a novel fusion antibacterial peptide with two shrimp penaeidins’ activities. Methods The chimeric gene of penaeidin-3 and penaeidin-4 was linked and amplifi ed through a linker by overlap-PCR. Prokaryotic expression plasmid pET-28a-P3P4 was constructed by inserting the gene into pET28a(+) and then transformed into E. coli BL21(DE3). After induced with 1 mmol/L IPTG at 37 ℃ for 4h or at 15 ℃ for 14 h, the expression products were analyzed by SDS-PAGE and the activities were shown by the inhibition zone method. Results Prokaryotic expression plasmid pET-28a-P3P4 was constructed. 18 % SDS-PAGE showed that the chimeric protein was expressed mainly as inclusion body with a molecular weight about 14.7×103 and the expression level at 15 ℃ was higher than that at 37 ℃. After renaturation, the chimeric protein showed antibacterial activity against both Bacillus subtilis and Vibrio alginolyticus. Conclusion A novel chimeric peptide with two shrimp penaeidins’ activities can be expressed in E. coli." @default.
- W1417509926 created "2016-06-24" @default.
- W1417509926 creator A5039213269 @default.
- W1417509926 creator A5055543356 @default.
- W1417509926 creator A5065274834 @default.
- W1417509926 creator A5068349486 @default.
- W1417509926 creator A5083956868 @default.
- W1417509926 creator A5087046547 @default.
- W1417509926 date "2009-01-01" @default.
- W1417509926 modified "2023-09-26" @default.
- W1417509926 title "Expression of chimeric gene of penaeidin-3 and penaeidin-4 in E. coli and its antibacterial activity assay." @default.
- W1417509926 hasPublicationYear "2009" @default.
- W1417509926 type Work @default.
- W1417509926 sameAs 1417509926 @default.
- W1417509926 citedByCount "0" @default.
- W1417509926 crossrefType "journal-article" @default.
- W1417509926 hasAuthorship W1417509926A5039213269 @default.
- W1417509926 hasAuthorship W1417509926A5055543356 @default.
- W1417509926 hasAuthorship W1417509926A5065274834 @default.
- W1417509926 hasAuthorship W1417509926A5068349486 @default.
- W1417509926 hasAuthorship W1417509926A5083956868 @default.
- W1417509926 hasAuthorship W1417509926A5087046547 @default.
- W1417509926 hasConcept C104317684 @default.
- W1417509926 hasConcept C111829193 @default.
- W1417509926 hasConcept C123894998 @default.
- W1417509926 hasConcept C150194340 @default.
- W1417509926 hasConcept C153911025 @default.
- W1417509926 hasConcept C170344550 @default.
- W1417509926 hasConcept C185592680 @default.
- W1417509926 hasConcept C22744801 @default.
- W1417509926 hasConcept C2776841996 @default.
- W1417509926 hasConcept C2777272437 @default.
- W1417509926 hasConcept C2779281246 @default.
- W1417509926 hasConcept C40767141 @default.
- W1417509926 hasConcept C505870484 @default.
- W1417509926 hasConcept C523546767 @default.
- W1417509926 hasConcept C54355233 @default.
- W1417509926 hasConcept C55493867 @default.
- W1417509926 hasConcept C86803240 @default.
- W1417509926 hasConceptScore W1417509926C104317684 @default.
- W1417509926 hasConceptScore W1417509926C111829193 @default.
- W1417509926 hasConceptScore W1417509926C123894998 @default.
- W1417509926 hasConceptScore W1417509926C150194340 @default.
- W1417509926 hasConceptScore W1417509926C153911025 @default.
- W1417509926 hasConceptScore W1417509926C170344550 @default.
- W1417509926 hasConceptScore W1417509926C185592680 @default.
- W1417509926 hasConceptScore W1417509926C22744801 @default.
- W1417509926 hasConceptScore W1417509926C2776841996 @default.
- W1417509926 hasConceptScore W1417509926C2777272437 @default.
- W1417509926 hasConceptScore W1417509926C2779281246 @default.
- W1417509926 hasConceptScore W1417509926C40767141 @default.
- W1417509926 hasConceptScore W1417509926C505870484 @default.
- W1417509926 hasConceptScore W1417509926C523546767 @default.
- W1417509926 hasConceptScore W1417509926C54355233 @default.
- W1417509926 hasConceptScore W1417509926C55493867 @default.
- W1417509926 hasConceptScore W1417509926C86803240 @default.
- W1417509926 hasIssue "9" @default.
- W1417509926 hasLocation W14175099261 @default.
- W1417509926 hasOpenAccess W1417509926 @default.
- W1417509926 hasPrimaryLocation W14175099261 @default.
- W1417509926 hasRelatedWork W2027359512 @default.
- W1417509926 hasRelatedWork W2338706024 @default.
- W1417509926 hasRelatedWork W2348496189 @default.
- W1417509926 hasRelatedWork W2348730693 @default.
- W1417509926 hasRelatedWork W2349654769 @default.
- W1417509926 hasRelatedWork W2350889661 @default.
- W1417509926 hasRelatedWork W2363346272 @default.
- W1417509926 hasRelatedWork W2365132954 @default.
- W1417509926 hasRelatedWork W2367641973 @default.
- W1417509926 hasRelatedWork W2367815219 @default.
- W1417509926 hasRelatedWork W2368849146 @default.
- W1417509926 hasRelatedWork W2371742534 @default.
- W1417509926 hasRelatedWork W2375346140 @default.
- W1417509926 hasRelatedWork W2379297578 @default.
- W1417509926 hasRelatedWork W2380208042 @default.
- W1417509926 hasRelatedWork W2384634207 @default.
- W1417509926 hasRelatedWork W2389758042 @default.
- W1417509926 hasRelatedWork W2389764293 @default.
- W1417509926 hasRelatedWork W2390193671 @default.
- W1417509926 hasRelatedWork W2391738441 @default.
- W1417509926 hasVolume "11" @default.
- W1417509926 isParatext "false" @default.
- W1417509926 isRetracted "false" @default.
- W1417509926 magId "1417509926" @default.
- W1417509926 workType "article" @default.