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- W1421303376 abstract "This chapter discusses the analysis of crystalline bacterial surface (S) layers by freeze-etching, metal shadowing, negative staining, and ultrathin sectioning. When using freeze-etching techniques for studying S-layers, the main purpose of the sampling, pretreatment, and cryofixation procedures is to preserve the regular array as closely as possible to that existing in vivo. Most S-layers completely cover the cell surface, leaving no gaps. Some organisms possess more than one S-layer. In such instances the fracturing process may separate adjacent layers. On rod-shaped cells S-layer patterns are generally uniform over large areas of the cylindrical part of the cells. Oblique and square lattices are usually arranged with one axis parallel to the long axis of the cell, but with some organisms a strain-specific skew angle may be observed. Freeze-etching is suitable for evaluating S-layers labeled with morphologically detectable marker molecules. Labeling with polycationic ferritin, a marker for negative surface charges, is used for probing the net charge of native and chemically modified S-layers." @default.
- W1421303376 created "2016-06-24" @default.
- W1421303376 creator A5020717643 @default.
- W1421303376 creator A5030094052 @default.
- W1421303376 creator A5052082810 @default.
- W1421303376 date "1988-01-01" @default.
- W1421303376 modified "2023-09-23" @default.
- W1421303376 title "2 Analysis of Crystalline Bacterial Surface Layers by Freeze-etching, Metal Shadowing, Negative Staining and Ultrathin Sectioning" @default.
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- W1421303376 doi "https://doi.org/10.1016/s0580-9517(08)70046-1" @default.
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