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- W1423223826 abstract "B67 Many human colorectal adenocarcinoma cell lines have been developed. However, anal canal squamous cell carcinoma (SCC) cell line has not yet been reported because this disease is uncommon. We have first established human anal canal SCC cell line designated SaTM-1 from primary culture of inguinal lymph node metastasis. Six sublines were cloned and isolated from SaTM-1 parental cells by the limiting dilution method. These lines were designated as SaTM-1A, -1B, -1C, -1D, -1E and -1F and maintained for over 25 months. Immunohistochemical analysis of both original tumor tissue and established culture cells showed cytokeratin-5 and p63 expression suggesting the characteristics of squamous cell carcinoma in all sublines. Their biological characteristics were studied including morphology, determination of doubling time, chemosensitivity to 5-Fu and CDDP, gene expression of growth factors and immunoblotting of proteins associated with lymphangiogenesis, and growth as xenograft in immunodeficient mice. All sublines were proliferated in multiple layers and observed adhesive and floating properties with an average doubling time of 24.5 hours. Cytotoxic effect of 5-Fu against all sublines were almost similar, although those of CDDP showed different behavior, such as the IC 50 of SaTM-1F cells for CDDP were 2.5-fold higher than SaTM-1B cells. They expressed vascular endothelial growth factor (VEGF) family and their receptors, such as VEGF-A, -B, VEGFR-1, -R3 and EGFR were expressed in all sublines, whereas VEGF-D and EGF were all not detected. Immunofluorescene analysis demonstrated SaTM-1A, -1B, -1D and -1F cells express Prox-1 and Podoplanin as lymphangiogenesis specific markers. SaTM-1 cells form tumor easily by subcutaneous injection, resulting that all sublines had tumorigenic property in immunodeficent mice. We first report the successful establishment and characterization of new anal canal SCC cell line SaTM-1, which may provide a beneficial cellular resource for investigating the biological behavior of human anal canal SCC." @default.
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- W1423223826 date "2007-11-15" @default.
- W1423223826 modified "2023-09-22" @default.
- W1423223826 title "Establishment and characterization of human anal canal carcinoma cell line (SaTM-1) derived from lymphnode metastasis" @default.
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