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- W142456368 abstract "This chapter presents a brief history of the histochemical methods for demonstrating sulfhydryl and disulfide groups in tissues. Histochemists and morphologists demonstrate the sites of thiols in tissues by various colorimetric methods. The chapter describes the rationale, methodology, and specificity of a recent histochemical method for protein-bound sulfhydryls utilizing a new reagent, 2,2-dihydroxy-6,6'-dinaphthyl disulfide as well as the use of this reagent in the histochemical demonstration of both sulfhydryls and disulfides or disulfides alone. The chapter discusses the effect of various methods of fixation on the histochemical demonstration of certain sulfhydryl- and disulfide-containing protein. It describes in detail attempts to modify the protein-bound sulfhydryl method for the demonstration of glutathione in addition to protein-bound sulfhydryls." @default.
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- W142456368 date "1954-01-01" @default.
- W142456368 modified "2023-09-27" @default.
- W142456368 title "Histochemical Experiments on Sulfhydryls and Disulfides" @default.
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- W142456368 doi "https://doi.org/10.1016/b978-1-4832-2900-3.50012-2" @default.
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