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- W1424955059 abstract "Publisher Summary This chapter describes a current and simple rapid method for sequencing long fragments of deoxyribonucleic acid (DNA) by combining the advantages of the two systems: (1) a systematic shortening of double-stranded DNA and (2) subcloning. In this method, a fragment of DNA carrying the origin of replication from a single-stranded phage, F1 or M13, is inserted into a plasmid to make a single-stranded:double-stranded bifunctional plasmid of the shortened fragments into sequencing vectors. The new family of bifunctional plasmid constructed is of pAA-PZ1, pAA-PZ2, pAA-PZ3, and pAA-PZ4. The chapter presents the methods in a simplified way for selecting the transposon-generated deletion plasmids of different sizes and for isolating the single-stranded plasmid DNA for DNA sequence analysis. The chapter discusses solutions, buffers, media, and sources of enzymes for the method. The chapter also discusses construction and properties of pAA-PZ plasmids, construction and property of E. coli strain AA102F', cloning of foreign DNA into pAA-PZ plasmids, and several related concepts and procedures." @default.
- W1424955059 created "2016-06-24" @default.
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- W1424955059 date "1987-01-01" @default.
- W1424955059 modified "2023-10-16" @default.
- W1424955059 title "[16] A new and simple rapid method for sequencing DNA" @default.
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- W1424955059 doi "https://doi.org/10.1016/0076-6879(87)55018-2" @default.
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