Matches in SemOpenAlex for { <https://semopenalex.org/work/W1426540051> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W1426540051 endingPage "58" @default.
- W1426540051 startingPage "51" @default.
- W1426540051 abstract "This chapter describes the methods for isolation of nuclei from cultured mammalian cells; conditions for preferential retention of selected histones. Histones serve as useful standards to assay for leakage of nuclear components as a function of the composition of the media used for cell lysis and isolation of nuclei. The chapter discusses the methods to study two kinds of histones: (1) lysine-rich and slightly lysine-rich histones, and (2) arginine-rich histones. None of the methods used for cell lysis prior to isolation of nuclei and chromatin conserves all the histones. The arginine-rich histones are poorly conserved by methods that use chelating agents, such as ethylenediaminetetraacetic acid (EDTA) or citrate, in the absence of appropriate concentrations of divalent cations. A neutral pH method that minimizes leakage of arginine-rich histones through the addition of 10 mM Mg 2+ and Ca 2+ has been developed." @default.
- W1426540051 created "2016-06-24" @default.
- W1426540051 creator A5057742255 @default.
- W1426540051 date "1978-01-01" @default.
- W1426540051 modified "2023-09-25" @default.
- W1426540051 title "Chapter 4 Methods for Isolation of Nuclei from Cultured Mammalian Cells; Conditions for Preferential Retention of Selected Histones" @default.
- W1426540051 cites W1482941043 @default.
- W1426540051 cites W1503396568 @default.
- W1426540051 cites W1596325941 @default.
- W1426540051 cites W1849202480 @default.
- W1426540051 cites W1965219854 @default.
- W1426540051 cites W1966115559 @default.
- W1426540051 cites W1996266432 @default.
- W1426540051 cites W2080321555 @default.
- W1426540051 doi "https://doi.org/10.1016/s0091-679x(08)61133-4" @default.
- W1426540051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/703622" @default.
- W1426540051 hasPublicationYear "1978" @default.
- W1426540051 type Work @default.
- W1426540051 sameAs 1426540051 @default.
- W1426540051 citedByCount "4" @default.
- W1426540051 crossrefType "book-chapter" @default.
- W1426540051 hasAuthorship W1426540051A5057742255 @default.
- W1426540051 hasConcept C178790620 @default.
- W1426540051 hasConcept C185592680 @default.
- W1426540051 hasConcept C2776016237 @default.
- W1426540051 hasConcept C2777468819 @default.
- W1426540051 hasConcept C515207424 @default.
- W1426540051 hasConcept C552990157 @default.
- W1426540051 hasConcept C55493867 @default.
- W1426540051 hasConcept C57409179 @default.
- W1426540051 hasConcept C64927066 @default.
- W1426540051 hasConcept C74884574 @default.
- W1426540051 hasConcept C83640560 @default.
- W1426540051 hasConcept C86803240 @default.
- W1426540051 hasConcept C95444343 @default.
- W1426540051 hasConceptScore W1426540051C178790620 @default.
- W1426540051 hasConceptScore W1426540051C185592680 @default.
- W1426540051 hasConceptScore W1426540051C2776016237 @default.
- W1426540051 hasConceptScore W1426540051C2777468819 @default.
- W1426540051 hasConceptScore W1426540051C515207424 @default.
- W1426540051 hasConceptScore W1426540051C552990157 @default.
- W1426540051 hasConceptScore W1426540051C55493867 @default.
- W1426540051 hasConceptScore W1426540051C57409179 @default.
- W1426540051 hasConceptScore W1426540051C64927066 @default.
- W1426540051 hasConceptScore W1426540051C74884574 @default.
- W1426540051 hasConceptScore W1426540051C83640560 @default.
- W1426540051 hasConceptScore W1426540051C86803240 @default.
- W1426540051 hasConceptScore W1426540051C95444343 @default.
- W1426540051 hasLocation W14265400511 @default.
- W1426540051 hasLocation W14265400512 @default.
- W1426540051 hasOpenAccess W1426540051 @default.
- W1426540051 hasPrimaryLocation W14265400511 @default.
- W1426540051 hasRelatedWork W1506160043 @default.
- W1426540051 hasRelatedWork W1509400502 @default.
- W1426540051 hasRelatedWork W1553350912 @default.
- W1426540051 hasRelatedWork W2003639282 @default.
- W1426540051 hasRelatedWork W2022599441 @default.
- W1426540051 hasRelatedWork W2040991523 @default.
- W1426540051 hasRelatedWork W2129029894 @default.
- W1426540051 hasRelatedWork W2142422993 @default.
- W1426540051 hasRelatedWork W2409141281 @default.
- W1426540051 hasRelatedWork W2569805444 @default.
- W1426540051 isParatext "false" @default.
- W1426540051 isRetracted "false" @default.
- W1426540051 magId "1426540051" @default.
- W1426540051 workType "book-chapter" @default.