Matches in SemOpenAlex for { <https://semopenalex.org/work/W142881930> ?p ?o ?g. }
- W142881930 abstract "Previous investigations have demonstrated that reactive oxygen species such as hydrogen peroxide (H2O2) have the ability to alter electrophysiological and mechanical properties of rat ventricular cardiac myocytes. However, despite the breadth of the literature, there is little definitive consensus on the cellular mechanisms. The purpose of this study, therefore, was to study the cellular mechanism of action of H2O2 and test whether H2O2-mediated affects were partially a result of reverse-mode Na/Ca exchanger (NCX) activity. Unloaded cell shortening, intracellular Ca transients, caffeine induced Ca transients, L-type Ca channel recordings, and action potential waveforms were recorded in the presence of combinations of different compounds including Cd, H2O2, and KB-R7943. H2O2 was found to cause significant positive inotropy by an increase in contractility of 80 ± 20 % (n=6) and an increased amplitude of Ca transients by 24 ± 14 % (n=8), relative to pre-treatment values. Interestingly, H2O2 caused an increase in contractility even in the presence of Cd block from 4 ± 1 % (n=9) to 15 ± 3 % (n=5) of resting cell length. Using caffeine pulse experiments to induce unloading of the sarcoplasmic reticulum (SR), we found that 100μM H2O2 did not significantly alter SR Ca load. Under control conditions, H2O2 significantly increased L-type Ca currents while this H2O2-induced increase was not observed in myocytes pretreated with Cd. Positive inotropy in the presence of H2O2 was blocked using 10μM KB-R7943, a selective reverse-mode inhibitor of the NCX. However, it was found that 10μM KB-R7943 alone altered action potential profile and suppressed normal contraction. Altogether, the major finding of this study is that H2O2 has the ability to" @default.
- W142881930 created "2016-06-24" @default.
- W142881930 creator A5065062850 @default.
- W142881930 date "2009-01-01" @default.
- W142881930 modified "2023-09-27" @default.
- W142881930 title "MODULATION OF CARDIAC MYOCYTE FUNCTION BY REACTIVE OXYGEN SPECIES" @default.
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