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- W1432877207 abstract "This chapter discusses the determination of nucleic acid base composition by chemical reactivity. By means of the spectral changes resulting from the difference of the reactivity of adenine and the other bases toward bromine reagents, the relative percentage of adenine can be estimated. Thus, a simple spectroscopic method for the determination of the base composition of DNA was realized. This method can be used for the determinations of base compositions of polynucleotides containing two different bases one of which is adenine. This method can be extended to the determination of the base composition of RNA if the RNA has the complementary base pairing relationship as in DNA. The relative amount of adenine in a DNA sample could be measured directly. The chapter discusses the determinations of base compositions of DNA and base compositions of polynucleotides containing two different bases one of which is adenine." @default.
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- W1432877207 date "1968-01-01" @default.
- W1432877207 modified "2023-10-16" @default.
- W1432877207 title "[107] The determination of nucleic acid base composition by chemical reactivity" @default.
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- W1432877207 doi "https://doi.org/10.1016/0076-6879(67)12131-9" @default.
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