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- W1433522883 abstract "This chapter describes the main features of the high-voltage technique and its application in particular to the problem of membrane turnover by exocytosis and endocytosis. Exposure to brief, intense electric fields provides a simple, rapid, and chemically clean method for gaining access to the interior of cells. Under certain conditions high-voltage techniques can be used to render the plasma membrane transiently permeable, whereas under other conditions the plasma membrane remains permeable for prolonged periods. Two methods have been used routinely to subject isolated cells to electric fields: (1) passing cells through a Coulter counter, and (2) discharging a capacitor through a cell suspension. The main disadvantage of using the Coulter-counter method is the length of time taken to harvest a worthwhile number of “leaky” cells. The two main disadvantages of the high-voltage technique are that accessibility is largely restricted to small molecules and that even for these molecules the rate of change in intracellular solute composition is dependent on the diameter of the cell and the number and size of the pores that have been created by exposure to the electric field." @default.
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- W1433522883 date "1983-01-01" @default.
- W1433522883 modified "2023-10-17" @default.
- W1433522883 title "[3] High-voltage techniques for gaining access to the interiot of cells: Application to the study of exocytosis and membrane turnover" @default.
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- W1433522883 doi "https://doi.org/10.1016/0076-6879(83)98136-3" @default.
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