Matches in SemOpenAlex for { <https://semopenalex.org/work/W1434517181> ?p ?o ?g. }
- W1434517181 endingPage "179" @default.
- W1434517181 startingPage "161" @default.
- W1434517181 abstract "Publisher Summary This chapter focuses on techniques for transforming Drosophila cell lines. The chapter shows how individual lines differ substantially in their transfectabilities by hydroxyapatite-DNA. They may also differ in their precise medium requirements, in their susceptibilities to the toxins used for selection, in superficial and changeable properties like surface adherence, and in the precise catalog of expressed genes. Drosophila cell lines can be maintained in a variety of media: Schneider's medium, D-22, and M3, all of which are now commercially available. The chapter emphasizes on the use of M3 because most cell lines adapt readily to growth in M3 supplemented with 5–10% fetal calf serum (FCS). Some lines require additional supplementation with a mixture of bactopeptone (BP) and extra yeast extract (YE). Because there is substantial clone-to-clone variation in the expression of a transformed plasmid, and because bulk selection of a transfected population generally leads to the retention of only the fastest-growing transformed clone within the starting population, it is frequently useful to clone cells just after transfection." @default.
- W1434517181 created "2016-06-24" @default.
- W1434517181 creator A5009393718 @default.
- W1434517181 creator A5016896546 @default.
- W1434517181 creator A5075853791 @default.
- W1434517181 date "1994-01-01" @default.
- W1434517181 modified "2023-10-13" @default.
- W1434517181 title "Chapter 9 Transformation Techniques for Drosophila Cell Lines" @default.
- W1434517181 cites W119642550 @default.
- W1434517181 cites W135858796 @default.
- W1434517181 cites W1554886997 @default.
- W1434517181 cites W1627615914 @default.
- W1434517181 cites W1781094001 @default.
- W1434517181 cites W1954706214 @default.
- W1434517181 cites W1954929088 @default.
- W1434517181 cites W1966985901 @default.
- W1434517181 cites W1967928127 @default.
- W1434517181 cites W1975771868 @default.
- W1434517181 cites W1981831710 @default.
- W1434517181 cites W1995583556 @default.
- W1434517181 cites W1996227893 @default.
- W1434517181 cites W1997115294 @default.
- W1434517181 cites W2000686528 @default.
- W1434517181 cites W2007602176 @default.
- W1434517181 cites W2022802536 @default.
- W1434517181 cites W2030229259 @default.
- W1434517181 cites W2034926683 @default.
- W1434517181 cites W2037123658 @default.
- W1434517181 cites W2037922547 @default.
- W1434517181 cites W2044568260 @default.
- W1434517181 cites W2048207102 @default.
- W1434517181 cites W2049422734 @default.
- W1434517181 cites W2058261722 @default.
- W1434517181 cites W2060640993 @default.
- W1434517181 cites W2061886886 @default.
- W1434517181 cites W2062655106 @default.
- W1434517181 cites W2063171821 @default.
- W1434517181 cites W2069811032 @default.
- W1434517181 cites W2084099638 @default.
- W1434517181 cites W2084520007 @default.
- W1434517181 cites W2085006948 @default.
- W1434517181 cites W2092576954 @default.
- W1434517181 cites W2097978662 @default.
- W1434517181 cites W2109664605 @default.
- W1434517181 cites W2111341106 @default.
- W1434517181 cites W2119300279 @default.
- W1434517181 cites W2127175258 @default.
- W1434517181 cites W2128147740 @default.
- W1434517181 cites W2150422177 @default.
- W1434517181 cites W2184304067 @default.
- W1434517181 cites W22014799 @default.
- W1434517181 cites W2503903664 @default.
- W1434517181 cites W4232896780 @default.
- W1434517181 cites W4249227535 @default.
- W1434517181 cites W65475208 @default.
- W1434517181 cites W842123435 @default.
- W1434517181 cites W2062397545 @default.
- W1434517181 doi "https://doi.org/10.1016/s0091-679x(08)60912-7" @default.
- W1434517181 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7707950" @default.
- W1434517181 hasPublicationYear "1994" @default.
- W1434517181 type Work @default.
- W1434517181 sameAs 1434517181 @default.
- W1434517181 citedByCount "68" @default.
- W1434517181 countsByYear W14345171812012 @default.
- W1434517181 countsByYear W14345171812013 @default.
- W1434517181 countsByYear W14345171812014 @default.
- W1434517181 countsByYear W14345171812015 @default.
- W1434517181 countsByYear W14345171812016 @default.
- W1434517181 countsByYear W14345171812017 @default.
- W1434517181 countsByYear W14345171812018 @default.
- W1434517181 countsByYear W14345171812020 @default.
- W1434517181 countsByYear W14345171812021 @default.
- W1434517181 countsByYear W14345171812022 @default.
- W1434517181 countsByYear W14345171812023 @default.
- W1434517181 crossrefType "book-chapter" @default.
- W1434517181 hasAuthorship W1434517181A5009393718 @default.
- W1434517181 hasAuthorship W1434517181A5016896546 @default.
- W1434517181 hasAuthorship W1434517181A5075853791 @default.
- W1434517181 hasConcept C104317684 @default.
- W1434517181 hasConcept C204241405 @default.
- W1434517181 hasConcept C2776041557 @default.
- W1434517181 hasConcept C54355233 @default.
- W1434517181 hasConcept C78458016 @default.
- W1434517181 hasConcept C86803240 @default.
- W1434517181 hasConceptScore W1434517181C104317684 @default.
- W1434517181 hasConceptScore W1434517181C204241405 @default.
- W1434517181 hasConceptScore W1434517181C2776041557 @default.
- W1434517181 hasConceptScore W1434517181C54355233 @default.
- W1434517181 hasConceptScore W1434517181C78458016 @default.
- W1434517181 hasConceptScore W1434517181C86803240 @default.
- W1434517181 hasLocation W14345171811 @default.
- W1434517181 hasLocation W14345171812 @default.
- W1434517181 hasOpenAccess W1434517181 @default.
- W1434517181 hasPrimaryLocation W14345171811 @default.
- W1434517181 hasRelatedWork W2086781068 @default.
- W1434517181 hasRelatedWork W2159009609 @default.
- W1434517181 hasRelatedWork W2323999364 @default.
- W1434517181 hasRelatedWork W4230568640 @default.