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• W1435363487 abstract "The anecdotal information that persisted for 100 years maintained that the only reservoir of toxigenic Vibrio cholerae O1, the epidemic strain of cholera, was the human intestinal tract and that this strain was taxonomically separate from V. cholerae non-O1, also known as “nonagglutinable vibrios” and found in aquatic, predominantly estuarine environments. The autochthonous nature of V. cholerae Ol is an important factor in the epidemiology of cholera and is significantly so in endemic areas. The first extensive studies of salinity and temperature relationships using microcosms were reported by Singleton and coworkers who used V. cholerae O1 strain LA4808, isolated during the 1978 El Tor cholera outbreak in Louisiana, and nine other clinical or environmental strains, including both O1 and non-O1 V. cholerae. Anwarul Huq and coworkers demonstrated that V. cholerae associated with planktonic live copepods survived longer, i.e., remained culturable, in laboratory microcosms than V. cholerae exposed to dead copepods or to Pseudoisochrysis sp., a blue-green alga used to feed live copepods. In this study, cells of V. cholerae were enumerated by plate count, and it was found that the organisms could be cultured on plates for up to 336 h. The study was carried out with divers in the Chesapeake Bay region of the United States and at the Black Sea off the coasts of Russia and Ukraine. V. cholerae O1 was isolated by culture and detected in seawater and from swabs of the ears, noses, and throats of divers by the fluorescent-antibody method when culture was unsuccessful." @default.
• W1435363487 created "2016-06-24" @default.
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• W1435363487 date "2014-04-08" @default.
• W1435363487 modified "2023-10-18" @default.
• W1435363487 title "Vibrios in the Environment: Viable but Nonculturable <i>Vibrio cholerae</i>" @default.
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• W1435363487 doi "https://doi.org/10.1128/9781555818364.ch9" @default.
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