FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1435710109> ?p ?o ?g. }

• W1435710109 endingPage "39" @default.
• W1435710109 startingPage "28" @default.
• W1435710109 abstract "FKBP22, an Escherichia coli-specific peptidyl-prolyl cis-trans isomerase, shows substantial homology with the Mip-like virulence factors. Mip-like proteins are homodimeric and possess a V-shaped conformation. Their N-terminal domains form dimers, whereas their C-terminal domains bind protein/peptide substrates and distinct inhibitors such as rapamycin and FK506. Interestingly, the two domains of the Mip-like proteins are separated by a lengthy, protease-susceptible α-helix. To delineate the structural requirement of this domain-connecting region in Mip-like proteins, we have investigated a recombinant FKBP22 (rFKBP22) and its three point mutants I65P, V72P and A82P using different probes. Each mutant harbors a Pro substitution mutation at a distinct location in the hinge region. We report that the three mutants are not only different from each other but also different from rFKBP22 in structure and activity. Unlike rFKBP22, the three mutants were unfolded by a non-two state mechanism in the presence of urea. In addition, the stabilities of the mutants, particularly I65P and V72P, differed considerably from that of rFKBP22. Conversely, the rapamycin binding affinity of no mutant was different from that of rFKBP22. Of the mutants, I65P showed the highest levels of structural/functional loss and dissociated partly in solution. Our computational study indicated a severe collapse of the V-shape in I65P due to the anomalous movement of its C-terminal domains. The α-helical nature of the domain-connecting region is, therefore, critical for the Mip-like proteins." @default.
• W1435710109 created "2016-06-24" @default.
• W1435710109 creator A5049785430 @default.
• W1435710109 creator A5050463530 @default.
• W1435710109 creator A5064519424 @default.
• W1435710109 creator A5080200652 @default.
• W1435710109 creator A5090189132 @default.
• W1435710109 date "2015-01-01" @default.
• W1435710109 modified "2023-09-27" @default.
• W1435710109 title "Proline substitutions in a Mip-like peptidyl-prolyl cis-trans isomerase severely affect its structure, stability, shape and activity" @default.
• W1435710109 cites W1553234807 @default.
• W1435710109 cites W1564719710 @default.
• W1435710109 cites W1612747316 @default.
• W1435710109 cites W1831229176 @default.
• W1435710109 cites W187801720 @default.
• W1435710109 cites W1931164952 @default.
• W1435710109 cites W1965295746 @default.
• W1435710109 cites W1968174488 @default.
• W1435710109 cites W1969317040 @default.
• W1435710109 cites W1970832803 @default.
• W1435710109 cites W1973111841 @default.
• W1435710109 cites W1974147209 @default.
• W1435710109 cites W1980500898 @default.
• W1435710109 cites W1990194768 @default.
• W1435710109 cites W1990279175 @default.
• W1435710109 cites W2007079396 @default.
• W1435710109 cites W2020583180 @default.
• W1435710109 cites W2023511357 @default.
• W1435710109 cites W2025336895 @default.
• W1435710109 cites W2026637052 @default.
• W1435710109 cites W2029667189 @default.
• W1435710109 cites W2030632688 @default.
• W1435710109 cites W2032059266 @default.
• W1435710109 cites W2038296759 @default.
• W1435710109 cites W2046875506 @default.
• W1435710109 cites W2047233626 @default.
• W1435710109 cites W2052749821 @default.
• W1435710109 cites W2053111993 @default.
• W1435710109 cites W2054066978 @default.
• W1435710109 cites W2054544606 @default.
• W1435710109 cites W2065931405 @default.
• W1435710109 cites W2068963648 @default.
• W1435710109 cites W2074964731 @default.
• W1435710109 cites W2078259224 @default.
• W1435710109 cites W2086382728 @default.
• W1435710109 cites W2087065403 @default.
• W1435710109 cites W2088301780 @default.
• W1435710109 cites W2089663290 @default.
• W1435710109 cites W2110040181 @default.
• W1435710109 cites W2111817477 @default.
• W1435710109 cites W2118233996 @default.
• W1435710109 cites W2120502960 @default.
• W1435710109 cites W2124026197 @default.
• W1435710109 cites W2128635872 @default.
• W1435710109 cites W2132589721 @default.
• W1435710109 cites W2139157185 @default.
• W1435710109 cites W2146788056 @default.
• W1435710109 cites W2148779562 @default.
• W1435710109 cites W2150352010 @default.
• W1435710109 cites W2152674241 @default.
• W1435710109 cites W2154170041 @default.
• W1435710109 cites W2154319440 @default.
• W1435710109 cites W2160090073 @default.
• W1435710109 cites W2165888313 @default.
• W1435710109 cites W2170067815 @default.
• W1435710109 cites W2170175152 @default.
• W1435710109 cites W2292488250 @default.
• W1435710109 cites W2323104068 @default.
• W1435710109 cites W4243773797 @default.
• W1435710109 cites W4293247451 @default.
• W1435710109 cites W76602793 @default.
• W1435710109 cites W1683286185 @default.
• W1435710109 doi "https://doi.org/10.1016/j.biopen.2015.07.001" @default.
• W1435710109 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5889476" @default.
• W1435710109 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29632827" @default.
• W1435710109 hasPublicationYear "2015" @default.
• W1435710109 type Work @default.
• W1435710109 sameAs 1435710109 @default.
• W1435710109 citedByCount "10" @default.
• W1435710109 countsByYear W14357101092016 @default.
• W1435710109 countsByYear W14357101092019 @default.
• W1435710109 countsByYear W14357101092020 @default.
• W1435710109 countsByYear W14357101092021 @default.
• W1435710109 crossrefType "journal-article" @default.
• W1435710109 hasAuthorship W1435710109A5049785430 @default.
• W1435710109 hasAuthorship W1435710109A5050463530 @default.
• W1435710109 hasAuthorship W1435710109A5064519424 @default.
• W1435710109 hasAuthorship W1435710109A5080200652 @default.
• W1435710109 hasAuthorship W1435710109A5090189132 @default.
• W1435710109 hasBestOaLocation W14357101091 @default.
• W1435710109 hasConcept C103408237 @default.
• W1435710109 hasConcept C104317684 @default.
• W1435710109 hasConcept C12554922 @default.
• W1435710109 hasConcept C143065580 @default.
• W1435710109 hasConcept C154472170 @default.
• W1435710109 hasConcept C16318435 @default.
• W1435710109 hasConcept C169627665 @default.
• W1435710109 hasConcept C176944494 @default.

• next