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- W1436612425 abstract "The chapter discusses the virus structure revealed by negative staining. General features of virus particles are described. The most efficient designs of protein containers are helical tubes and icosahedral shells. In fact, it is evident that a very large number of virus particles have either helical or icosahedral symmetry. The chapter discusses the procedures for improving contrast of biological materials and in particular of virus particles. The techniques discussed include Shadow Casting, Replica Methods, Positive Staining of Isolated Particles, Thin Sectioning, and Negative Staining Methods. It is becoming more and more obvious that means must be devised for relating structure of virus particles with their function. This can be done only in the crudest possible way until robust quantitative methods have been introduced for electron microscopy. Methods of particle counting have been briefly discussed in relation to negative staining. The negative staining technique does, however, enable morphological differentiation and it is thus possible to estimate total numbers of structurally distinct particles." @default.
- W1436612425 created "2016-06-24" @default.
- W1436612425 creator A5017802888 @default.
- W1436612425 creator A5036275737 @default.
- W1436612425 date "1964-01-01" @default.
- W1436612425 modified "2023-10-18" @default.
- W1436612425 title "Virus Structure Revealed by Negative Staining" @default.
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