FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W143773279> ?p ?o ?g. }

• W143773279 abstract "Efficient and selective delivery and retrieval of cargo to polarized plasma membrane domains are essential for the maintenance of epithelial cell function. My studies focused on events involved in protein endocytosis from and delivery to the apical surface.Endocytosis is a multi-step process that is crucial for cells to take up nutrients and modulate receptor density. Temporal synthesis and degradation of the lipid phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] is critical for endocytosis to proceed. PtdIns(4,5)P2 is generated by three differentially localized phosphatidylinositol 4-phosphate 5-kinase (PIP5KI) isoforms (α, β, and γ). My studies examined the requirement for PtdIns(4,5)P2 in apical and basolateral endocytosis in polarized kidney epithelial cells by testing the effects of overexpressing individual PIP5KI isoforms. I found that PtdIns(4,5)P2 synthesis by PIP5KIβ is rate limiting for apical but not basolateral endocytosis, and that this is likely due to changes in coated pit maturation at the apical plasma membrane. The selective modulation of apical endocytosis by PtdIns(4,5)P2 has potential consequences on the maintenance of kidney cell function. Maintaining apical and basolateral membrane domain composition requires selective vesicle fusion and is mediated by Soluble N-ethylmaleimide-sensitive factor Attachment protein Receptor (SNARE) proteins. A number of pathways exist for the delivery of newly synthesized proteins to these surface domains and the post-Golgi vesicle SNAREs, or VAMPs, that mediate fusion in these pathways have not been identified. I sought to determine the role of VAMP7 in these pathways. VAMP7 expressed in polarized Madin Darby canine kidney cells colocalized primarily with LAMP2-positive compartments, and changes in VAMP7 levels modulated lysosome size, consistent with the known function of VAMP7 in lysosomal delivery. Surprisingly, VAMP7 knockdown had no effect on apical delivery of numerous cargoes tested but did decrease the length and frequency of primary cilia and disrupted cyst morphology. The effects of VAMP7 depletion on ciliogenesis and cystogenesis are not directly linked to the disruption of lysosomal function, as cilia lengths and cyst morphology were unaffected in a MDCK lysosomal storage disorder model. Together, my data suggest that VAMP7 plays an essential role in ciliogenesis and cystogenesis. This is the first study implicating an R-SNARE in ciliogenesis and cystogenesis." @default.
• W143773279 created "2016-06-24" @default.
• W143773279 creator A5022268819 @default.
• W143773279 date "2013-05-29" @default.
• W143773279 modified "2023-09-23" @default.
• W143773279 title "MODULATION OF ENDOCYTIC AND EXOCYTIC TRAFFIC IN POLARIZED EPITHELIAL CELLS" @default.
• W143773279 cites W1510276228 @default.
• W143773279 cites W1514917008 @default.
• W143773279 cites W1516832564 @default.
• W143773279 cites W1527146728 @default.
• W143773279 cites W1527391598 @default.
• W143773279 cites W1575705959 @default.
• W143773279 cites W1893137636 @default.
• W143773279 cites W1963503129 @default.
• W143773279 cites W1963573377 @default.
• W143773279 cites W1963676316 @default.
• W143773279 cites W1964881184 @default.
• W143773279 cites W1965522502 @default.
• W143773279 cites W1965940344 @default.
• W143773279 cites W1966621469 @default.
• W143773279 cites W1967737783 @default.
• W143773279 cites W1968255775 @default.
• W143773279 cites W1970987547 @default.
• W143773279 cites W1971060242 @default.
• W143773279 cites W1971775244 @default.
• W143773279 cites W1972204116 @default.
• W143773279 cites W1974056290 @default.
• W143773279 cites W1974646226 @default.
• W143773279 cites W1974679439 @default.
• W143773279 cites W1975437274 @default.
• W143773279 cites W1977951612 @default.
• W143773279 cites W1978324226 @default.
• W143773279 cites W1980205113 @default.
• W143773279 cites W1980529461 @default.
• W143773279 cites W1982270633 @default.
• W143773279 cites W1982532032 @default.
• W143773279 cites W1982750125 @default.
• W143773279 cites W1983921682 @default.
• W143773279 cites W1985708338 @default.
• W143773279 cites W1987246337 @default.
• W143773279 cites W1988741834 @default.
• W143773279 cites W1992186441 @default.
• W143773279 cites W1992189978 @default.
• W143773279 cites W1992215989 @default.
• W143773279 cites W1996896272 @default.
• W143773279 cites W1997349759 @default.
• W143773279 cites W1997576450 @default.
• W143773279 cites W1997671401 @default.
• W143773279 cites W1998172466 @default.
• W143773279 cites W1998796212 @default.
• W143773279 cites W1999476309 @default.
• W143773279 cites W2000302106 @default.
• W143773279 cites W2000994188 @default.
• W143773279 cites W2001248812 @default.
• W143773279 cites W2003383185 @default.
• W143773279 cites W2006467843 @default.
• W143773279 cites W2006884799 @default.
• W143773279 cites W2007184758 @default.
• W143773279 cites W2007202084 @default.
• W143773279 cites W2009703499 @default.
• W143773279 cites W2010629357 @default.
• W143773279 cites W2010896265 @default.
• W143773279 cites W2012022940 @default.
• W143773279 cites W2013064662 @default.
• W143773279 cites W2014201243 @default.
• W143773279 cites W2016923195 @default.
• W143773279 cites W2017250842 @default.
• W143773279 cites W2017633690 @default.
• W143773279 cites W2019990719 @default.
• W143773279 cites W2020050184 @default.
• W143773279 cites W2021710660 @default.
• W143773279 cites W2022037546 @default.
• W143773279 cites W2022447364 @default.
• W143773279 cites W2023707309 @default.
• W143773279 cites W2023843317 @default.
• W143773279 cites W2024316397 @default.
• W143773279 cites W2025022118 @default.
• W143773279 cites W2025722012 @default.
• W143773279 cites W2027610252 @default.
• W143773279 cites W2027639590 @default.
• W143773279 cites W2029018698 @default.
• W143773279 cites W2031057786 @default.
• W143773279 cites W2031062227 @default.
• W143773279 cites W2031461843 @default.
• W143773279 cites W2032009989 @default.
• W143773279 cites W2033210506 @default.
• W143773279 cites W2033344378 @default.
• W143773279 cites W2033371578 @default.
• W143773279 cites W2034146282 @default.
• W143773279 cites W2035805790 @default.
• W143773279 cites W2038544607 @default.
• W143773279 cites W2038678925 @default.
• W143773279 cites W2040003712 @default.
• W143773279 cites W2040483955 @default.
• W143773279 cites W2040654496 @default.
• W143773279 cites W2041844278 @default.
• W143773279 cites W2042181541 @default.
• W143773279 cites W2042324409 @default.
• W143773279 cites W2042773138 @default.
• W143773279 cites W2043365258 @default.

• next