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- W1437974741 abstract "Publisher Summary Caenorhabditis elegans provides a system in which many biological processes involving microtubules, including multicellular processes such as development and nerve function, can be studied in the context of the living animal. C. elegans contains several structurally distinct microtubule types, including 11 protofilament microtubules, 13 protofilament microtubules in the A-subfiber of ciliary outer doublet microtubules, and 15 protofilament microtubules in some specialized sensory nerve processes that detect touch. Microtubules purified by reassembly from C. elegans are composed of between 8 and 13 protofilaments. They also contain a variety of associated proteins with interesting properties. This chapter covers methods for growing C. elegans in quantities sufficient for biochemical studies and for the isolation of microtubules and microtubule-associated proteins (MAPs) from C. elegans. An adult C. elegans is only 1 mm long so the manipulation of single animals is most easily performed under a dissecting microscope. It is easy to keep cultures free of contaminating bacteria and fungus by using sterile technique." @default.
- W1437974741 created "2016-06-24" @default.
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- W1437974741 date "1991-01-01" @default.
- W1437974741 modified "2023-09-27" @default.
- W1437974741 title "[24] Isolation of microtubules, adligin, and other microtubule-associated proteins from Caenorhabditis elegans" @default.
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- W1437974741 doi "https://doi.org/10.1016/0076-6879(91)96026-n" @default.
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