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- W1439062022 abstract "The cystic fibrosis transmembrane conductance regulator, CFTR, is expressed at the apical membranes of many epithelial cells, where it mediates cAMP-stimulated anion secretion. In thyroid, CFTR may indirectly and/or directly furnish iodide for hormone synthesis. CFTR functionally interacts with many membrane transporters via PSD-Discs large homologous-ZO-1 (PDZ) domain-containing scaffolding proteins, thereby forming macromolecular transport complexes. SLC5A8 is an electrogenic, sodium-coupled monocarboxylate transporter that also is expressed at thyrocyte apical membranes. CFTR and SLC5A8 contain the identical PDZ domain interaction motif; preliminary studies utilizing overexpression systems indicate a role for PDZ adaptor proteins in linking CFTR and SLC5A8. The present studies use the Fisher rat thyroid cell line, FRTL-5, to test the hypothesis that PDZ proteins facilitate CFTR/SLC5A8 interactions in an endogenously expressing system. Expression of CFTR (~150 kDa), SLC5A8 (~68 kDa) and NHERF-1 (~50 kDa) in total FRTL-5 lysates was confirmed by immunoblot analysis using anti-CFTR (M3A7 and Cystic Fibrosis Foundation Therapeutics (CFFT) 660), anti-SLC5A8 (Abcam) and anti-NHERF-1 (Alomone) antibodies. Anti-SLC5A8-immunoprecipitated (IPd) fractions of FRTL-5 lysates reacted with both CFFT 660 and anti-NHERF-1. Fractions IPd using anti-CFTR (M3A7) were probed with anti-SLC5A8 and anti-NHERF-1. M3A7-IPd fractions showed positive reactivity with anti-SLC5A8, but NHERF-1 was not detected. These data suggest that although CFTR and SLC5A8 associate in FRTL-5 cells, the participation of NHERF-1 is not required. Support: NIH/NIGMS Award 1R15GM101674-01A1 (to PF); Office of the Director, NIH Award Number T35OD010979." @default.
- W1439062022 created "2016-06-24" @default.
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- W1439062022 date "2015-04-01" @default.
- W1439062022 modified "2023-09-27" @default.
- W1439062022 title "CFTR and SLC5A8 Associate in Thyroid Epithelial Cells" @default.
- W1439062022 doi "https://doi.org/10.1096/fasebj.29.1_supplement.969.14" @default.
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